典型文献
A highly selective fluorescent probe for real-time imaging of UDP-glucuronosyltransferase 1A8 in living cells and tissues
文献摘要：
Uridine diphosphate(UDP)-glucuronosyl-transferases(UGTs)are enzymes involved in the biotrans-formation of important endogenous compounds such as steroids,bile acids,and hormones as well as exogenous substances including drugs,environmental toxicants,and carcinogens.Here,a novel fluorescent probe BDMP was developed based on boron-dipyrromethene(BODIPY)with high sensitivity for the detection of UGT1A8.The glucuronidation of BDMP not only exhibited a red-emission wavelength(λex/λem=500/580 nm),but also displayed an excellent UGTlA8-dependent fluorescence signal with a good linear relationship with UGT1A8 concentration.Based on this perfect biocompatibility and cell permeability,BDMP was successfully used to image endogenous UGT1A8 in human cancer cell lines(LoVo and HCT15)in real time.In addition,BDMP could also be used to visualize UGT1A8 in tumor tissues.These results suggested that BDMP is a promising molecular tool for the investigation of UGT1 A8-mediated physiological function in humans.
文献关键词：
中图分类号：
作者姓名：
Mingyue Zhu;Zhenhao Tian;Lingling Jin;Xiaokui Huo;Chao Wang;Jingnan Cui;Yan Tian;Xiangge Tian;Lei Feng
作者机构：
College of Pharmacy,School of Medicine,Key Laboratory of Elemene Class Anti-Cancer Chinese Medicines,Engineering Laboratory of Development and Application of Traditional Chinese Medicines,Hangzhou Normal University,Hangzhou 311121,China;Dalian Key Laboratory of Metabolic Target Characterization and Traditional Chinese Medicine Intervention,College of Pharmacy,College of Integrative Medicine,Dalian Medical University,Dalian 116044,China;School of Life Sciences,Northwestern Polytechnical University,Xi'an 710072,China;State Key Laboratory of Fine Chemicals,Dalian University of Technology,Dalian 116024,China
文献出处：
引用格式：
[1]Mingyue Zhu;Zhenhao Tian;Lingling Jin;Xiaokui Huo;Chao Wang;Jingnan Cui;Yan Tian;Xiangge Tian;Lei Feng-.A highly selective fluorescent probe for real-time imaging of UDP-glucuronosyltransferase 1A8 in living cells and tissues)[J].化学科学与工程前沿,2022(01):103-111
A类：
glucuronosyltransferase,1A8,glucuronosyl,biotrans,BDMP,UGT1A8,UGTlA8
B类：
highly,selective,fluorescent,probe,real,imaging,UDP,living,cells,tissues,Uridine,diphosphate,transferases,UGTs,are,enzymes,involved,formation,important,endogenous,compounds,such,steroids,bile,acids,hormones,well,exogenous,substances,including,drugs,environmental,toxicants,carcinogens,Here,novel,was,developed,boron,dipyrromethene,BODIPY,sensitivity,detection,glucuronidation,not,only,exhibited,red,emission,wavelength,but,also,displayed,excellent,dependent,fluorescence,signal,good,linear,relationship,concentration,Based,this,perfect,biocompatibility,permeability,successfully,used,image,cancer,lines,LoVo,HCT15,In,addition,could,visualize,tumor,These,results,suggested,that,promising,molecular,tool,investigation,mediated,physiological,function,humans
AB值：
0.587349
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
