Nuclear RNA export into the cytoplasm is one of the key steps in protein expression to realize biological functions.Despite the broad availability of nucleic acid dyes,tracking and quantifying the highly dynamic process of RNA export in live cells is challenging.When dye-labeled RNA enters the cytoplasm,the dye molecules are released upon degradation of the RNA,allowing them to re-enter the cell nucleus.As a result,the ratio between the dye exported with RNA into the cytoplasm and the portion staying inside the nucleus cannot be determined.To address this common limitation,we report the design of a smart probe that can only check into the nucleus once.When adding to cells,this probe rapidly binds with nuclear RNAs in live cells and reacts with intrinsic H2S.This reaction not only activates the fluorescence for RNA tracking but also changes the structure of probe and consequently its intracellular localization.After disassociating from exported RNAs in cytoplasm,the probe preferentially enters lysosomes rather than cell nucleus,enabling real-time quantitative measurement of nuclear RNA exports.Using this probe,we successfully evaluated the effects of hormones and cancer drugs on nuclear RNA export in live cells.Interestingly,we found that hormones inhibiting RNA exports can partially offset the effect of chemother-apy.

Jie Shen;Juan Chen;Dong Wang;Zhengjie Liu;Guangmei Han;Bianhua Liu;Mingyong Han;Ruilong Zhang;Guodong Liu;Zhongping Zhang

School of Chemistry and Chemical Engineering,Information Materials and Intelligent Sensing Laboratory of Anhui Province,and Institute of Physical Science and Information Technology,Anhui University,Hefei 230601,China;Key Lab of Photovoltaic and Energy Conservation Materials,Institute of Solid State Physics,HFIPS,Chinese Academy of Sciences,Hefei 230031,China;Key Laboratory of Structure and Functional Regulation of Hybrid Materials(Anhui University),Ministry of Education,Hefei 230601,China;School of Life and Health Sciences,Anhui Science and Technology University,Chuzhou 233100,China

中国化学快报（英文版）

[1]Jie Shen;Juan Chen;Dong Wang;Zhengjie Liu;Guangmei Han;Bianhua Liu;Mingyong Han;Ruilong Zhang;Guodong Liu;Zhongping Zhang-.Real-time quantification of nuclear RNA export using an intracellular relocation probe)[J].中国化学快报（英文版）,2022(08):3865-3868

disassociating,chemother

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