In the race to deliver clean water to communities through potable water reuse,disinfec-tion and water quality assessment are and will continue to be fundamental factors.There are over 700 disinfection byproducts(DBPs)in water;evaluating each compound is practi-cally impossible and very time consuming.A bioanalytical approach could be an answer to this challenge.In this work,the response of four major classes of DBPs toward mitochon-drial membrane potential(ΔΨm)and cytoplasmic adenosine triphosphate(C-ATP)was in-vestigated with human carcinoma(HepG2)cells.Within 90 min of cell exposure,only the haloacetic acid(HAA)mixture caused a cytotoxic response as measured by C-ATP.All four groups(haloacetonitriles(HANs),trihalomethanes(THMs),nitrosamines(NOAs),and HAAs)responded well to Δvm,R2＞0.70.Based on the half-maximum concentration that evoked a 50％response in Δvm,the response gradient was HANs＞＞HAAs～THM＞NOAs.The inhibition of the Δvm by HANs is driven by dibromoacetonitrile(DBAN),while dichloroace-tonitrile(DCAN)did not cause a significant change in the Δvm at less than 2000 μM.A mixture of HANs exhibited an antagonistic behavior on the Δvm compared to individual compounds.If water samples are concentrated to increase HAN concentrations,especially DBAN,then ΔΨm could be used as a biomonitoring tool for DBP toxicity.

George William Kajjumba;Rachael E.Bokota;Matias Attene-Ramos;Erica J.Marti

Department of Civil and Environmental Engineering,University of Nevada,Las Vegas,NV,USA;Department of Environmental and Occupational Health,Milken Institute School of Public Health,George Washington University,Washington DC,USA

环境科学学报（英文版）

[1]George William Kajjumba;Rachael E.Bokota;Matias Attene-Ramos;Erica J.Marti-.Evaluation of disinfection byproducts for their ability to affect mitochondrial function)[J].环境科学学报（英文版）,2022(07):295-304

disinfec,haloacetic,haloacetonitriles,NOAs,dibromoacetonitrile,DBAN,dichloroace,tonitrile,biomonitoring

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