The number and quality of sperm are decreased due to bisphenol A (BPA) exposure, an endocrine-disrupting chemical on the male reproductive system, especially in advanced paternal age (APA). Procyanidin C-1 (PCY-1), an antioxidant from grape seed (Vitis vinifera L.), has demonstrated anti-viral, anti-melanogenic and immunostimulatory effects. Therefore, this study aims to determine the effects of PCY-1 intervention on sperm parameters, testis morphological changes, serum testosterone, oestradiol, and luteinizing hormone (LH) concentrations and the expression of apoptotic (Bax and Bcl-2) and mitochondria-related (Mfn1 and Opa1) genes in BPA-exposed aged mice. Results revealed that PCY-1 intervention improves aged male fertility in BPA-exposed conditions by decreasing abnormal sperms percentage and increasing spermatogenic cell diameter and epithelial height. PCY-1 also decreased oestradiol, and increased LH and testosterone levels. The gene expression of Bax was significantly down-regulated by PCY-1 intervention. In contrast, Bcl-2 was substantially up-regulated. Expression of Mfn1 and Opa1 genes were also significantly up-regulated in the PCY intervention group. Hence, it is demonstrated that PCY-1 was able to mitigate the adverse effects of BPA on reproductive parameters of aged mice. Collectively, we postulated that PCY-1 has a potential role in protecting the ageing male reproductive system against the damaging impacts of BPA.

Mastura Abd Malek;Razif Dasiman;Nor-Ashikin Mohamed Noor Khan;Sofee Mohamed-Akhlak;Mohd-Hafizi Mahmud

Maternofetal and Embryo Research Group(MatE),Faculty of Medicine,Universiti Teknologi MARA,Selangor Branch,Sungai Buloh Campus,47000 Sungai Buloh,Selangor,Malaysia;Faculty of Health Sciences,Universiti Teknologi MARA,Selangor Branch,Puncak Alam Campus,42300 Puncak Alam,Selangor,Malaysia

食品科学与人类健康（英文）

[1]Mastura Abd Malek;Razif Dasiman;Nor-Ashikin Mohamed Noor Khan;Sofee Mohamed-Akhlak;Mohd-Hafizi Mahmud-.The protective effects of procyanidin C-1 on bisphenol A-induced testicular dysfunction in aged mice)[J].食品科学与人类健康（英文）,2022(04):965-974

Procyanidin,melanogenic

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