Male infertility has evolved from a common reproductive system disease to a major social issue.Youjing granule(YG)is a Chinese medicinal material used as a therapy method for tonifying the kidneys and removing dampness due to its pathogenic char-acteristics.YG has been shown to regulate sperm quality in clinical trials,but the underlying mechanism is not fully understood.The present study was aimed to explore the protective effects and mechanism of action of YG on male reproductive system damage caused by methyl methane sulfonate(MMS).We first established an infertility model of rats through oral administration of MMS and then treated with YG.To determine the effect of YG,spermatogenesis,microvascular density,and secretory function of Leydig cells and Sertoli cells in rats were assessed.Spermatogonial stem cells(SSCs)were co-cultured with mouse embryo fibroblast(MEF)cells as an in vitro cell model before exposure to serum containing YG.Furthermore,the proliferation and apoptosis of SSCs were measured.Res-ults indicated that YG increased the expression of self-renewal and proliferation-related molecules such as glial cell line derived neuro-trophic factor(GDNF)and fibroblast growth factor-2(FGF2),and improved the quality of sperm and the proliferation of SSCs.In con-clusion,YG may protect spermatogenetic function of rats through regulating the proliferation and self-renewal of SSCs.

JIANG Xu-Ping;SUN Yao-Xiang;QIAO Bin;ZHU Wen-Jiao;CHU Yu-Jiao;SUN Miao-Miao;TANG Rui-Jie;MA Tie-Liang;TANG Zhi-An

Department of Traditional Chinese Medicine,the Affiliated Yixing Clinical School of Medical School of Yangzhou University,Yixing 214200,China;Central Laboratory,the Affiliated Yixing Hospital of Jiangsu University,Yixing 214200,China;Department of Rehabilitation,the People's Hospital of Daxinganling Region,Daxinganling 165000,China;School of Medicine,School of Integrated Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing 210023,China

中国天然药物

[1]JIANG Xu-Ping;SUN Yao-Xiang;QIAO Bin;ZHU Wen-Jiao;CHU Yu-Jiao;SUN Miao-Miao;TANG Rui-Jie;MA Tie-Liang;TANG Zhi-An-.Youjing granules ameliorate spermatogenesis in rats through regulating the prolifereation of spermatogonial stem cells)[J].中国天然药物,2022(08):580-588

Youjing,prolifereation,Spermatogonial,spermatogenetic

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