典型文献
Single intratesticular injection of blood- serum- derived exosomes can potentially alleviate testopathy following testicular torsion
文献摘要：
Background : Testicular torsion (TT) is an acute inflammatory process leading to male infertility. Today, anti-i nflammatory effects of exosomes derived from blood serum are used in various laboratory procedures. In the present study, the anti-i nflammatory effects of blood- serum- derived exosomes in treatment of acute inflammation following TT in mice were evaluated. Materials and Methods : Eighteen male mice were grouped as healthy control, TT, and TT + exosome. TT was induced surgically, and exosomes were extracted from blood serum and administrated by a single intratesticular injection (10 IU). Malondialdehyde (MDA) and Griess assays were used to evaluate the level of oxidative stress. Sperm indices, testosterone (Tes), and apoptotic gene expression (p- 53, Bcl2, and Caspase- 3) were also assessed. H&E and immunohistochemistry (IHC) stainings were used for histopathological investigations. Data analysis was applied by SPSS (v.19) software. Results : Oxidative stress and apoptotic genes expression were increased significantly ( p < 0.05) in TT group compared with control. Sperm parameters and Tes were significantly increased, and expression of apoptotic genes was significantly reduced in TT + exosome group ( p < 0.05). Conclusion : Since the blood- serum- derived exosomes have anti- inflammatory features, the intratesticular application of blood- serum- derived exosomes can be used clinically in acute phase of orchitis following TT to inhibit testicular inflammation.
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作者姓名：
Mona Keivan;Fatemeh Mansouri Torghabeh;Samira Davoodi;Shima Moradi Maryamneghari;Reza Dadfar
作者机构：
Member of Research Committee,Medical School,Kermanshah University of Medical Sciences,Kermanshah,Iran;Department of Physiology Sciences,Medical School,Mashhad University of Medical Sciences,Mashhad,Iran;Department of Anatomical Sciences,Medical School,Kermanshah University of Medical Sciences,Kermanshah,Iran;Department of Anatomical Sciences,Faculty of Medicine,AJA University of Medical Sciences,Tehran,Iran
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引用格式：
[1]Mona Keivan;Fatemeh Mansouri Torghabeh;Samira Davoodi;Shima Moradi Maryamneghari;Reza Dadfar-.Single intratesticular injection of blood- serum- derived exosomes can potentially alleviate testopathy following testicular torsion)[J].动物模型与实验医学（英文）,2022(04):362-368
A类：
intratesticular,testopathy,nflammatory,Tes,stainings,orchitis
B类：
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AB值：
0.455789
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