Background : TDCPP is one of the major chemical of organophosphate flame re-tardants (OPFRs) that has been detected ubiquitously in both the environment and biota. Previously we observed that it influenced the concentrations of sex and thy-roid hormones in a sex- dependent pattern, leading to reproductive impairments after short- term exposure in zebrafish. Here we investigate the consequences of longer- term exposure to TDCPP on the hypothalamic- pituitary- gonad (HPG), hypothalamic- pituitary-i nterrenal (HPI), and hypothalamic- pituitary- thyroid (HPT) axes of zebrafish ( Danio rerio ). Methods : A 120- day exposure test to 0.005, 0.05 and 0.5 mg/L TDCPP was initiated with fertilized eggs. Sex steroid hormones in the treated fishes were measured and transcriptional changes were analyzed. Results : In female fish, exposure to TDCPP resulted in increases in plasma cortisol, follicle stimulating hormone (FSH), luteinizing hormone (LH), 17β- estradiol (E2), cor-tisol, thyroxine (T4), and triiodothyronine (T3). Transcription of most target genes along HPG, HPI and HPT axes were increased by the exposure. While in male fish the exposure led to decreases in cortisol, FSH, LH, T4, T3, testosterone (T), and 11- ketotestosterone (11- KT). Transcription of genes along HPG, HPI and HPT axes, especially steroidogenic genes, were inhibited in male zebrafish. While, E2/T or E2/11- KT ratio was increased in both female and females. The sex- dependent changes in hormones might be due to differential responses to TDCPP induced stresses. An increase in cortisol level coincided with increases in E2 and THs in female fish, while in males decreases in cortisol as well as T, 11- KT and THs were observed. Long- term exposure to TDCPP at very low (μg/L) concentrations could disrupt hormone balances in a sex dependent way. Conclusion : This study revealed that TDCPP could affect endocrine axes – HPG, HPI and HPT – in zebrafish, and impair zebrafish development.

Xiaoshan Liu;Xiaoxun Lu;Jiabin Hong;Jing Zhang;Juntong Lin;Mengzhu Jiang;Qian Liu;Kyungho Choi;Jingjing Zhang

School of Public Health,Dongguan Key Laboratory of Environmental Medicine,Guangdong Medical University,Guangdong,China;Graduate School of Public Health and Institute of Health and Environment,Seoul National University,Seoul,Korea;Affiliated Hospital of Guangdong Medical University&Key Laboratory of Zebrafish Model for Development and Disease, Guangdong Medical University,Zhanjiang,China;The Marine Biomedical Research Institute of Guangdong Zhanjiang,Zhanjiang, Guangdong,China

动物模型与实验医学（英文）

[1]Xiaoshan Liu;Xiaoxun Lu;Jiabin Hong;Jing Zhang;Juntong Lin;Mengzhu Jiang;Qian Liu;Kyungho Choi;Jingjing Zhang-.Effects of long- term exposure to TDCPP in zebrafish ( Danio rerio ) – Alternations of hormone balance and gene transcriptions along hypothalamus- pituitary axes)[J].动物模型与实验医学（英文）,2022(03):239-247

Alternations,tardants,nterrenal,tisol,ketotestosterone

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