Background : Nicotine administration can generate severe oxidative stress and lipid peroxidation. Royal jelly, with its antioxidant properties, acts as a scavenger of reac-tive oxygen species. This study describes the apitherapy effects of royal jelly on tes-ticular damage following nicotine administration. Methods : Forty- eight male BALB/c mice were divided into 8 groups ( n = 6): saline, 3 different doses of royal jelly (100, 150, and 200 mg/kg body weight (BW) per day), nicotine (1.5 mg/kg), and 3 different groups of Nic + Roy (1.5 mg/kg of Nic + 100, 150, and 200 mg/kg BW per day of royal jelly). Nicotine was administrated intraperi-toneally, and royal jelly was prescribed orally for 10 consecutive days. Serum levels of hormones (testosterone, luteinizing hormone, and follicle- stimulating hormone), total antioxidant capacity, nitric oxide (NO) status, malondialdehyde levels, sperm DNA fragmentation, sperm parameters, histopathological changes (H&E staining), im-munohistochemistry against apoptotic proteins, and gene expression of Bcl - 2 , p53 , Caspase - 3 , and Nrf2 (real- time PCR) were assessed to evaluate the molecular and his-tological changes. Results : Hormone levels, sperm parameters, and status of antioxidants were de-creased significantly ( p < .05) following nicotine administration. Moreover, royal jelly treatment normalized hormonal and antioxidant characteristics, decreased apoptotic gene expression, increased Nfr2 gene expression, and restored histopathological al-teration to the physiological status significantly ( p < .05). Conclusion : Royal jelly upregulates the antioxidant status, inhibits the mitochondrial- dependent apoptosis pathway, and increases the rate of proliferation. This thera-peutic agent effectively protected the testis against nicotine- associated damages by antioxidant and anti- apoptotic effects.

Mahsa Nazar-Zadeh;Cyrus Jalili;Amene Nikgoftar Fathi;Ali Ghanbari;Mitra Bakhtiari

Department of Anatomical Sciences,Medical School,Kermanshah University of Medical Sciences,Kermanshah,Iran;Medical Biology Research Center,Kermanshah University of Medical Sciences,Kermanshah,Iran;Department of Anatomical Sciences,Medical School,Arak University of Medical Sciences,Arak,Iran

动物模型与实验医学（英文）

[1]Mahsa Nazar-Zadeh;Cyrus Jalili;Amene Nikgoftar Fathi;Ali Ghanbari;Mitra Bakhtiari-.Royal- jelly- based apitherapy can attenuate damages to male reproductive parameter following nicotine administration)[J].动物模型与实验医学（英文）,2022(02):133-140

apitherapy,intraperi,toneally,munohistochemistry,tological,Nfr2,teration

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