Objective:To systematically explore the effect and mechanism of melastomatis dodecandri herba(Melastoma dodecandrum Lour.) in the treatment of hepatitis based on network pharmacology.Method:We evaluated the hepatoprotective effects of M.dodecandrum in concanavalin A (Con A)-induced hepatitis in mice by assessing survival rate,histological analysis,serum transaminases,and related cytokines.Then the mechanism of action was predicted by a network pharmacology-based strategy.Based on the results,we measured the hepatic expression of related genes at mRNA level and proteins related to the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) and nuclear factor-kappa B (NF-κB) pathways.Results:Our study results clearly demonstrated that M.dodecandrum pretreatment significantly allevi-ated liver injury.This was demonstrated by an increase in survival rate,decreased severity of liver damage,and reduced serum transaminase levels compared with those in the Con A group.Moreover,M.dodecandrum significantly reduced the serum levels of tumor necrosis factor-α,interleukin-6,and interferon-y and increased the liver levels of superoxide dismutase,which indicated that M.dodecandrum exhibits anti-inflammatory and antioxidant activities.On the basis of network phar-macology,50 nodes were selected as major hubs based on their topological importance.Pathway enrichment analyses indicated that the putative targets of M.dodecandrum mostly participate in various pathways associated with the anti-inflammation response,which implies the underlying mechanism by which M.dodecandrum acts on hepatitis.Real-time fluorescent quantitative PCR analysis showed that M.dodecandrum downregulates the mRNA expression of interleukin-6,Toll-like receptor 7,interleukin-1 receptor-associated kinase-4,NF-κB and tumor necrosis factor-α in liver tissues.Western blotting showed that M.dodecandrum pretreatment protected against inflammation through activating the PI3K-Akt pathway by upregulating phosphorylated Akt (p-Akt) expression and suppressing NF-κB activation by inhibiting the phosphorylation of IKK,IκBα,and p65.Conclusion:The present work demonstrated the hepatoprotective effects of M.dodecandrum by regu-lating the PI3K/Akt and NF-κB pathways in Con A-induced mice,which provide insights into the treat-ment of hepatitis using M.dodecandrum.

Jinfeng wang;Linyuan Wang;Zhihao Zhang;Min Wu;Wenting Fei;Zhihui Yang;Jianjun Zhang

School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing,102488,China;School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing,102488,China;School of Traditional Chinese Pharmacy,Chino Pharmaceutical University,Nanjing,211198,Chino;Medical College,Yangzhou University,Yangzhou,225001,China;Department of Emergency Medicine,University of Florida,Gainesville,32611,USA

中医科学杂志（英文）

[1]Jinfeng wang;Linyuan Wang;Zhihao Zhang;Min Wu;Wenting Fei;Zhihui Yang;Jianjun Zhang-.Elucidation of the hepatoprotective effect and mechanism of Melastoma dodecandrum Lour.based on network pharmacology and experimental validation)[J].中医科学杂志（英文）,2022(01):47-58

Elucidation,dodecandrum,melastomatis,dodecandri,transaminases

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