The tryptophan (Trp)-derived plant secondary metabolites, including camalexin, 4-hydroxy-indole-3-carbonylnitrile, and indolic glucosino-late (IGS), show broad-spectrum antifungal ac-tivity. However, the distinct regulations of these metabolic pathways among different plant spe-cies in response to fungus infection are rarely studied. In this study, our results revealed that WRKY33 directly regulates IGS biosynthesis, notably the production of 4-methoxyindole-3-ylmethyl glucosinolate (4MI3G), conferring re-sistance to Alternaria brassicicola, an important pathogen which causes black spot in Brassica crops. WRKY33 directly activates the expression of CYP81F2, IGMT1, and IGMT2 to drive side-chain modification of indole-3-ylmethyl glucosi-nolate (I3G) to 4MI3G, in both Arabidopsis and Chinese kale (Brassica oleracea var. alboglabra Bailey). However, Chinese kale showed a more severe symptom than Arabidopsis when infected by Alternaria brassicicola. Comparative analyses of the origin and evolution of Trp metabolism indicate that the loss of camalexin biosynthesis in Brassica crops during evolution might at-tenuate the resistance of crops to Alternaria brassicicola. As a result, the IGS metabolic pathway mediated by WRKY33 becomes es-sential for Chinese kale to deter Alternaria brassicicola. Our results highlight the differential regulation of Trp-derived camalexin and IGS bi-osynthetic pathways in plant immunity between Arabidopsis and Brassica crops.

Han Tao;Huiying Miao;Lili Chen;Mengyu Wang;Chuchu Xia;Wei Zeng;Bo Sun;Fen Zhang;Shuqun Zhang;Chuanyou Li;Qiaomei Wang

Department of Horticulture,Zhejiang University,Hangzhou 310058,Zhejiang,China;State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province,Institute of Virology and Biotechnology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;College of Horticulture,Sichuan Agricultural University,Chengdu 611130,China;Division of Biochemistry,Interdisciplinary Plant Group,University of Missouri,Columbia 65211,Missouri,USA;State Key Laboratory of Plant Genomics,National Centre for Plant Gene Research(Beijing),Institute of Genetics and Developmental Biology,the Chinese Academy of Sciences,Beijing 100101,China

植物学报（英文版）

[1]Han Tao;Huiying Miao;Lili Chen;Mengyu Wang;Chuchu Xia;Wei Zeng;Bo Sun;Fen Zhang;Shuqun Zhang;Chuanyou Li;Qiaomei Wang-.WRKY33-mediated indolic glucosinolate metabolic pathway confers resistance against Alternaria brassicicola in Arabidopsis and Brassica cropsOO)[J].植物学报（英文版）,2022(05):1007-1019

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