典型文献
BoALG10,an α-1,2 glycosyltransferase,plays an essential role in maintaining leaf margin shape in ornamental kale
文献摘要：
The morphological diversity of leaf margin shapes is an identifying characteristic of many plant species.In our previous work,BoALG10(α-1,2 glycosyltransferase)was predicted to be a key regulator of leaf margin shape in ornamental kale(Brassica oleracea var.acephala).An alanine and a leucine residue in the conserved domain of the smooth-margined S0835 were replaced by an aspartate and a phenylalanine,respectively,in the corresponding positions of the feathered-margined F0819.However,the expression pattern and function of this gene remain unclear.Here,we examined the expression patterns of BoALG10 using quantitative real-time PCR,and found that statistically significant differences in expression existed between F0819 and S0835 in nine developmental stages.The BoALG10 protein localized to the endoplasmic reticulum.The function of BoALG10 was then examined using complementary mutant assays.The overexpression strains phenocopied the smooth leaf margin after introduction of BoALG10S0835 into the feathered-margined inbred line F0819.Simultaneously,irregular dissections appeared in the leaf margins of knockout mutants KO-1 and KO-2,which were generated by CRISPR/Cas9 technology from the smooth-margined inbred line S0835.Microscopic observation showed that the leaf margin cells of the smooth-margined plants S0835 and OE-3 were arranged regularly,while the cells of the feathered-margined plants F0819 and KO-1 were of inconsistent size and distributed in an irregular manner,particularly around the indentations of the leaf.This elucidation of BoALG10 function provides a novel insight into the morphological regulation of leaf margin shape.
文献关键词：
中图分类号：
作者姓名：
Xin Feng;Xinru Yang;Meiqin Zhong;Xin Li;Pengfang Zhu
作者机构：
College of Forestry,Shenyang Agricultural University,Shenyang 110866,China;Key Laboratory of Forest Tree Genetics,Breeding and Cultivation of Liaoning Province,Shenyang 110866,China
文献出处：
引用格式：
[1]Xin Feng;Xinru Yang;Meiqin Zhong;Xin Li;Pengfang Zhu-.BoALG10,an α-1,2 glycosyltransferase,plays an essential role in maintaining leaf margin shape in ornamental kale)[J].园艺研究（英文）,2022(01):380-389
A类：
BoALG10,kale,margined,S0835,F0819,phenocopied,BoALG10S0835
B类：
glycosyltransferase,plays,essential,role,maintaining,leaf,ornamental,morphological,diversity,shapes,identifying,characteristic,many,species,In,our,previous,work,was,predicted,key,regulator,Brassica,oleracea,var,acephala,An,leucine,residue,conserved,domain,smooth,were,replaced,by,aspartate,phenylalanine,respectively,corresponding,positions,feathered,However,function,this,remain,unclear,Here,examined,patterns,using,quantitative,real,found,that,statistically,significant,differences,existed,between,developmental,stages,protein,localized,endoplasmic,reticulum,then,complementary,assays,overexpression,strains,after,introduction,into,inbred,line,Simultaneously,irregular,dissections,appeared,margins,knockout,mutants,KO,which,generated,CRISPR,Cas9,technology,from,Microscopic,observation,showed,cells,plants,OE,arranged,regularly,while,inconsistent,size,distributed,manner,particularly,around,indentations,This,elucidation,provides,novel,insight,regulation
AB值：
0.460628
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