典型文献
BrAN contributes to leafy head formation by regulating leaf width in Chinese cabbage(Brassica rapa L.ssp.pekinensis)
文献摘要：
Leafy head is an important agronomic trait that determines the yield and quality of Chinese cabbage.The molecular mechanism underlying heading in Chinese cabbage has been the focus of research,and wide leaves are a prerequisite for leafy head formation.In our study,two allelic leafy heading-deficient mutants(lhd1 and lhd2)with narrow leaf phenotypes were screened in an ethyl methanesulfonate mutagenized population from a heading Chinese cabbage double haploid line'FT'.Genetic analysis revealed that the mutant trait was controlled by a recessive nuclear gene,which was found to be BraA10g000480.3C by MutMap and Kompetitive allele-specific PCR analyses.As BraA10g000480.3C was the ortholog of ANGUSTIFOLIA in Arabidopsis,which has been found to regulate leaf width by controlling cortical microtubule arrangement and pavement cell shape,we named it BrAN.BrAN in mutant lhd1 carried an SNP(G to A)on intron 2 that co-segregated with the mutant phenotype,and disrupted the exon-intron splice junction generating intron retention and a putative truncated protein.BrAN in mutant lhd2 carried an SNP(G to A)on exon 4 leading to a premature stop codon.The ectopic overexpression of BrAN restored normal leaf phenotype due to abnormal cortical microtubule arrangement and pavement cell shape in the Arabidopsis an-t1 mutant.However,transformation of Bran did not rescue the an-t1 phenotype.These results indicate that BrAN contributes to leafy head formation of Chinese cabbage.
文献关键词：
中图分类号：
作者姓名：
Yue Xin;Chong Tan;Che Wang;Yanji Wu;Shengnan Huang;Yue Gao;Lu Wang;Nan Wang;Zhiyong Liu;Hui Feng
作者机构：
Liaoning Key Laboratory of Genetics and Breeding for Cruciferous Vegetable Crops,College of Horticulture,Shenyang Agricultural University,Shenyang 110866,China;College of Bioscience and Biotechnology,Shenyang Agricultural University,Shenyang 110866,China
文献出处：
引用格式：
[1]Yue Xin;Chong Tan;Che Wang;Yanji Wu;Shengnan Huang;Yue Gao;Lu Wang;Nan Wang;Zhiyong Liu;Hui Feng-.BrAN contributes to leafy head formation by regulating leaf width in Chinese cabbage(Brassica rapa L.ssp.pekinensis))[J].园艺研究（英文）,2022(01):390-401
A类：
BrAN,Leafy,lhd1,lhd2,mutagenized,BraA10g000480,ANGUSTIFOLIA,Bran
B类：
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AB值：
0.4644
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