典型文献
Transcriptome analysis reveals the differential regulatory effects of red and blue light on nitrate metabolism in pakchoi (Brassica campestris L.)
文献摘要：
Pakchoi (Brassica campestris L. ssp. chinensis) is an important leafy vegetable. Various light spectra, especially red and blue light, play vital roles in the regulation of nitrate metabolism. Information on the effects of red and blue light on nitrate metabolism at the transcriptome level in pakchoi is still limited, so this study used RNA sequencing technology to explore this molecular mechanism. Through pairwise comparisons with white LED light, 3939 and 5534 differentially expressed genes (DEGs) were identified under red and blue light, respectively. By Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analyses, these unigenes were found to be involved in nitrate assimilation, plant–pathogen interaction, biosynthesis of secondary metabolites, and phenylpropanoid biosynthesis. The differential effects of light spectra on the nitrate concentration and metabolism-related enzyme activities were also confirmed at the physiological level. Several signal transduction modules, including Crys/Phys-COP1-HY5/HY5-like, were found to be involved in red and blue light-induced nitrate metabolism, and the transcript levels for this complex were consistent with the observed degree of nitrate assimilation. The expression patterns of 15 randomly selected DEGs were further validated using qPCR. Taken together, the results of this study could help improve our understanding of light spectrum-regulated nitrate metabolism in pakchoi at the transcriptome level.
文献关键词：
中图分类号：
作者姓名：
FAN Xiao-xue;BIAN Zhong-hua;SONG Bo;XU Hai
作者机构：
Institute of Vegetable Crops/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,P.R.China;Photobiology Research Center,Institute of Urban Agriculture,Chinese Academy of Agricultural Sciences,Chengdu 610200,P.R.China
文献出处：
引用格式：
[1]FAN Xiao-xue;BIAN Zhong-hua;SONG Bo;XU Hai-.Transcriptome analysis reveals the differential regulatory effects of red and blue light on nitrate metabolism in pakchoi (Brassica campestris L.))[J].农业科学学报（英文）,2022(04):1015-1027
A类：
pakchoi,Pakchoi,Crys
B类：
Transcriptome,analysis,reveals,regulatory,effects,red,blue,light,nitrate,metabolism,Brassica,campestris,ssp,chinensis,important,leafy,vegetable,Various,spectra,especially,play,vital,roles,regulation,Information,transcriptome,still,limited,this,study,used,sequencing,technology,explore,molecular,mechanism,Through,pairwise,comparisons,white,LED,differentially,expressed,DEGs,were,identified,respectively,By,Kyoto,Encyclopedia,Genes,Genomes,Ontology,analyses,these,unigenes,found,be,involved,assimilation,plant,pathogen,interaction,biosynthesis,secondary,metabolites,phenylpropanoid,concentration,related,enzyme,activities,also,confirmed,physiological,Several,signal,transduction,modules,including,Phys,COP1,HY5,like,induced,levels,complex,consistent,observed,degree,expression,patterns,randomly,selected,further,validated,using,qPCR,Taken,together,results,could,help,improve,our,understanding,spectrum,regulated
AB值：
0.52498
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