The soil-borne necrotrophic fungus Rhizoctonia solani is one of destructive fungi causing severe yield losses in various important crops. However, the host defense mechanisms against the invasion of this pathogen are poorly understood. In this study, we employed an iTRAQ-based quantitative proteomic approach to investigate host proteins responsive to R. solani using the resistant rice cultivar YSBR1. As a whole, we identified 319 differentially accumulated proteins (DAPs) after inoculation of rice plants with R. solani. Functional categorization analysis indicates that these DAPs cover a broad range of functions. Notably, a substantial portion of the DAPs are involved in cell redox homeostasis, carbohydrate metabolism, and phenylpropanoid biosynthesis, or belong to pathogenesis-related proteins, indicating that these processes/proteins play important roles in host defense against R. solani. Interestingly, all of the DAPs involved in photosynthesis and chlorophyll biosynthetic processes, and part of the DAPs involved in phenylpropanoid biosynthesis, show reduced accumulation after R. solani infection, suggesting that R. solani probably inhibits host photosynthetic system and phenylpropanoid biosynthesis to facilitate infection and colonization. In conclusion, our results provide both valuable resources and new insights into the molecular mechanisms underlying rice and R. solani interaction.

FENG Zhi-ming;GAO Peng;ZHAO Jian-hua;WANG Guang-da;ZHANG Hui-min;CAO Wen-lei;XUE Xiang;ZHANG Ya-fang;MA Yu-yin;HUA Rong;CHEN Zong-xiang;CHEN Xi-jun;HU Ke-ming;ZUO Shi-min

Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding,Agricultural College,Yangzhou University,Yangzhou 225009,P.R.China;Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province,Yangzhou University,Yangzhou 225009,P.R.China;Jiangsu Hongqi Seed Stock Co.,Ltd.,Taizhou 225311,P.R.China;Yangzhou Polytechnic College,Yangzhou 225000,P.R.China;Joint International Research Laboratory of Agriculture and Agri-Product Safety,Ministry of Education of China/Institutes of Agricultural Science and Technology Development,Yangzhou University,Yangzhou 225009,P.R.China

农业科学学报（英文）

[1]FENG Zhi-ming;GAO Peng;ZHAO Jian-hua;WANG Guang-da;ZHANG Hui-min;CAO Wen-lei;XUE Xiang;ZHANG Ya-fang;MA Yu-yin;HUA Rong;CHEN Zong-xiang;CHEN Xi-jun;HU Ke-ming;ZUO Shi-min-.iTRAQ-based quantitative proteomics analysis of defense responses triggered by the pathogen Rhizoctonia solani infection in rice)[J].农业科学学报（英文）,2022(01):139-152

necrotrophic,YSBR1

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