A poor biocompatibility and bioactivity of invasive materials remains major problems for biomaterial-based therapy.In this study,we introduced gelatin scaffolds carrying both bone morphogenetic protein-2(BMP-2)biomimetic peptide and vascular endothelial growth factor-165(VEGF)that achieved controlled release,cell attachment,proliferation and differentiation.To promote osteogenesis with VEGF,we de-signed the BMP-2 biomimetic peptide that comprised BMP-2 core sequence oligopeptide(SSVPT),phos-phoserine,and synthetic cell adhesion factor(RGDS).In vitro cell experiments,the scaffold was conducive to the adhesion and proliferation of rat bone marrow mesenchymal stem cells(rBMSCs).The micro-CT 3D reconstruction of the rat cranial bone defect model showed that bone regeneration patterns occurred from one side edge towards the center area implanted with the prepared cryogel,and tissue section stain-ing analysis demonstrated that the scaffold with double-growth factor can synergistically accelerate bone regeneration.These findings suggested that the obtained gelatin cryogel could serve as a cell-responsive platform for biomaterial-based nonbearing bone repair.

Lili Wang;Long Chen;Jiping Wang;Liying Wang;Chenyu Gao;Bo Li;Yuanzheng Wang;Jun Wu;Changyun Quan

Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instruments,School of Biomedical Engineering,Sun Yat-sen University,Shenzhen 518107,China;Department of Orthopedics,Guizhou Provincial People's Hospital,Guiyang 550000,China;School of Public Health,Yale University,New Haven,CT 06510,United States;Department of Biomedical Engineering,Johns Hopkins University,Baltimore,MD 21218,United States

中国化学快报（英文版）

[1]Lili Wang;Long Chen;Jiping Wang;Liying Wang;Chenyu Gao;Bo Li;Yuanzheng Wang;Jun Wu;Changyun Quan-.Bioactive gelatin cryogels with BMP-2 biomimetic peptide and VEGF:A potential scaffold for synergistically induced osteogenesis)[J].中国化学快报（英文版）,2022(04):1956-1962

SSVPT,phoserine,RGDS,nonbearing

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