Numerous studies highlight advantages of electrospun scaffolds in bone tissue engineering,in which cellular behavior is tightly affected by fiber topographical cues of scaffolds.However,the classic elec-trospinning setup limits a desired presentation of biomimetic fibrous microenvironments that sense mechanosignaling and regulate stem cell behavior.The aims of this study were to fabricate advanced as-spun scaffolds presenting tree-like microfiber/nanonet networks and to evaluate their regulatory poten-tials on behavior of human mesenchymal stem cells(hMSCs)and bone regeneration.Here we developed a novel electrospinning setup that allowed the presentation of patterned Trunk microfibers(TMF)and/or branched nanonet fibers(BNnFs)in biomimetic fibrous scaffolds.As the cellular mechanisms,anisotropic-hierarchical topography of TMF controlled behavior of hMSCs through focal adhesion formation and Yes-associated protein(YAP)induction,whereas BNnF disturbed such mechanosensing responses in the cells.The fiber microenvironment-related expression and nuclear localization of YAP were.also correlated with the potentials of as-spun scaffolds to enhance osteogenic differentiation of the hMSCs and alveolar bone defect healing in an animal model.Collectively,this study provides an advanced approach of the modi-fied electrospinning setup for presentation of biomimetic fibrillar microenvironments in as-spun scaffolds along with their application in stem cell behavior regulation and regenerative tissue engineering.

Jeong In Kim;Ju Yeon Kim;Sung-Ho Kook;Jeong-Chae Lee

Cluster for Craniofacial Development and Regeneration Research,Institute of Oral Biosciences and School of Dentistry,Jeonbuk National University,Jeonju 54896,Republic of Korea;Department of Bionanosystem Engineering,Jeonbuk National University,Jeonju 54896,Republic of Korea;Department of Bioactive Material Sciences,Research Center of Bioactive Materials,Jeonbuk National University,Jeonju 54896,Republic of Korea

材料科学技术（英文版）

[1]Jeong In Kim;Ju Yeon Kim;Sung-Ho Kook;Jeong-Chae Lee-.A novel electrospinning method for self-assembled tree-like fibrous scaffolds:Microenvironment-associated regulation of MSC behavior and bone regeneration)[J].材料科学技术（英文版）,2022(20):52-70

trospinning,mechanosignaling,nanonet,BNnFs,BNnF

novel,electrospinning,method,self,assembled,tree,like,fibrous,scaffolds,Microenvironment,associated,regulation,behavior,bone,regeneration,Numerous,studies,highlight,advantages,electrospun,tissue,engineering,which,cellular,tightly,affected,by,topographical,cues,However,classic,setup,limits,desired,presentation,biomimetic,microenvironments,that,sense,regulate,stem,aims,this,study,were,fabricate,advanced,presenting,networks,evaluate,their,regulatory,human,mesenchymal,cells,hMSCs,Here,developed,allowed,patterned,Trunk,microfibers,TMF,branched,mechanisms,anisotropic,hierarchical,topography,controlled,through,focal,adhesion,formation,Yes,protein,YAP,induction,whereas,disturbed,such,mechanosensing,responses,expression,nuclear,localization,also,correlated,potentials,enhance,osteogenic,differentiation,alveolar,defect,healing,animal,model,Collectively,provides,approach,modi,fied,fibrillar,along,application,regenerative

0.509903