The biological performance of Ti-6Al-4V implant is primarily determined by their surface properties.However,traditional surface modification methods,such as acid etching,hardly make improvement in their osseointegration ability and antibacterial capacity.In this study,we prepared a multi-scale compos-ite structure coated with zinc oxide(ZnO)on Ti-6Al-4V implant by an innovative technology of two-step laser processing combined with solution-assistant.Compared with the acid etching method,the physic-ochemical properties of surface significantly improved.The in vitro results showed that the particular dimension of micro-nano structure and the multifaceted nature of ZnO synergistically affected MC3T3-E1 osteogenesis and bacterial activities:(1)The surface morphology showed a'contact guidance'effect on cell arrangement,which was conducive to the adhesion of filopodia and cell spreading,and the osteoge-nesis level of MC3T3-E1 was enhanced due to the release of zinc ions(Zn2+);(2)the characterization of bacterial response revealed that periodic nanostructures and Zn2+released could cause damage to the cell wall of E.coli and reduce the adhesion and aggregation of S.aureus.In conclusion,the modified surface showed a synergistic effect of physical topography and chemical composition,making this a promising method and providing new insight into bone defect repairment.

Yi Wan;Zihe Zhao;Mingzhi Yu;Zhenbing Ji;Teng Wang;Yukui Cai;Chao Liu;Zhanqiang Liu

Key Laboratory of High Efficiency and Clean Manufacturing,School of Mechanical Engineering,Shandong University,Jinan 250061,China;Centre of Micro/Nano Manufacturing Technology(MNMT-Dublin),School of Mechanical and Materials Engineering,University College Dublin,Dublin D04 KW52,Ireland;School of Engineering Sciences in Chemistry,Biotechnology and Health,KTH Royal,Institute of Technology,Stockholm,Stockholm S-10044,Sweden;Department of Oral and Maxilofacial Surgery,Qilu Hospital of Shandong University,Jinan 250012,China;Department of Oral Surgery,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine; College of Stomatology,Shanghai Jiao Tong University,National Center for Stomatology; National Clinical Research Center for Oral Diseases;Shanghai Key Laboratory of Stomatology, Shanghai 200011,China

材料科学技术（英文版）

[1]Yi Wan;Zihe Zhao;Mingzhi Yu;Zhenbing Ji;Teng Wang;Yukui Cai;Chao Liu;Zhanqiang Liu-.Osteogenic and antibacterial ability of micro-nano structures coated with ZnO on Ti-6Al-4V implant fabricated by two-step laser processing)[J].材料科学技术（英文版）,2022(36):240-252

filopodia,osteoge,Zn2+released

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