Novel bioactive injectable composites based on biopolymeric hydrogels reinforced with insulin-functionalized silica particles were synthesized.The insulin(INS)was immobilized on the surface of amine-modified silica particles employing covalent attachment by EDC/NHS chemistry and via electro-static interaction.The resulting formulations were examined for the morphology(SEM),chemical compo-sition(FTIR,XPS)as well as protein content.To facilitate the injectability and support the bone regener-ation,developed particles were dispersed in biopolymeric sol composed of collagen,chitosan and lysine-modified hyaluronic acid and crosslinked with genipin.By means of rheological study,the sol-gel in situ transition of obtained systems was verified.It was found in vitro study that MG-63 cells cultured on the developed composites exhibit significantly higher alkaline phosphatase(ALP)activity,compared to the pristine hydrogel.Furthermore,the biomineralization ability in the simulated body fluid(SBF)model was also demonstrated.Our findings suggest that proposed herein novel hydrogel-based composites might be the promising formulation for regeneration of bone defects,especially as a less-cost effective sup-port/alternative for BMP-2 systems.

Aleksandra Krajcer;Joanna Klara;Wojciech Horak;Joanna Lewandowska-?ańcucka

Faculty of Chemistry,Jagiellonian University,Gronostajowa 2,Kraków 30-387,Poland;AGH University of Science and Technology,Faculty of Mechanical Engineering and Robotics,Department of Machine Design and Technology,Al.Mickiewicza 30,Kraków 30-059,Poland

材料科学技术（英文版）

[1]Aleksandra Krajcer;Joanna Klara;Wojciech Horak;Joanna Lewandowska-?ańcucka-.Bioactive injectable composites based on insulin-functionalized silica particles reinforced polymeric hydrogels for potential applications in bone tissue engineering)[J].材料科学技术（英文版）,2022(10):153-163

biopolymeric,regener

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