典型文献
An automated nucleic acid detection platform using digital microfluidics with an optimized Cas12a system
文献摘要：
Outbreaks of both influenza virus and the novel coronavirus SARS-CoV-2 are serious threats to human health and life.It is very important to establish a rapid,accurate test with large-scale detection potential to prevent the further spread of the epidemic.An optimized RPA-Cas12a-based platform combined with digital microfluidics(DMF),the RCD platform,was established to achieve the automated,rapid detection of influenza viruses and SARS-CoV-2.The probe in the RPA-Casl2a system was optimized to produce maximal fluorescence to increase the amplification signal.The reaction droplets in the platform were all at the microliter level and the detection could be accomplished within 30 min due to the effective mixing of droplets by digital microfluidic technology.The whole process from amplification to recognition is completed in the chip,which reduces the risk of aerosol contamination.One chip can contain multiple detection reaction areas,offering the potential for customized detection.The RCD platform demonstrated a high level of sensitivity,specificity(no false positives or negatives),speed(≤30 min),automation and multiplexing.We also used the RCD platform to detect nucleic acids from influenza patients and COVID-19 patients.The results were consistent with the findings of qPCR.The RCD platform is a one-step,rapid,highly sensitive and specific method with the advantages of digital microfluidic technology,which circumvents the shortcomings of manual op-eration.The development of the RCD platform provides potential for the isothermal automatic detection of nucleic acids during epidemics.
文献关键词：
中图分类号：
作者姓名：
Zhen Sun;Kang-Feng Lin;Ze-Hang Zhao;Yang Wang;Xin-Xin Hong;Jian-Guang Guo;Qing-Yu Ruan;Lian-Yu Lu;Xiao Li;Rui Zhang;Chao-Yong Yang;Bo-An Li
作者机构：
State Key Laboratory of Cellular Stress Biology,Innovation Center for Cell Signaling Network and Engineering Research Center of Molecular Diagnostics of the Ministry of Education,School of Life Sciences,Xiamen University,Xiamen 361005,China;MOE Key Laboratory of Spectrochemical Analysis&Instrumentation,Key Laboratory of Chemical Biology of Fujian Province,State Key Laboratory of Physical Chemistry of Solid Surfaces,Collaborative Innovation Center of Chemistry for Energy Materials,Department of Chemical Engineering,Department of Chemical Biology,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China;Department of Thoracic Surgery,The First Affiliated Hospital,Xiamen University,Xiamen 361005,China
文献出处：
引用格式：
[1]Zhen Sun;Kang-Feng Lin;Ze-Hang Zhao;Yang Wang;Xin-Xin Hong;Jian-Guang Guo;Qing-Yu Ruan;Lian-Yu Lu;Xiao Li;Rui Zhang;Chao-Yong Yang;Bo-An Li-.An automated nucleic acid detection platform using digital microfluidics with an optimized Cas12a system)[J].中国科学:化学（英文版）,2022(03):630-640
A类：
Outbreaks,Casl2a,microliter
B类：
An,automated,nucleic,detection,platform,using,digital,microfluidics,optimized,Cas12a,system,both,influenza,novel,coronavirus,SARS,CoV,serious,threats,human,health,life,It,very,important,rapid,accurate,test,large,scale,potential,prevent,further,spread,RPA,combined,DMF,RCD,was,established,achieve,viruses,probe,produce,maximal,fluorescence,increase,amplification,signal,reaction,droplets,were,all,level,could,accomplished,within,due,effective,mixing,by,technology,whole,process,from,recognition,completed,chip,which,reduces,risk,aerosol,contamination,One,can,contain,areas,offering,customized,demonstrated,sensitivity,specificity,false,positives,negatives,speed,automation,multiplexing,We,also,used,acids,patients,results,consistent,findings,qPCR,one,step,highly,sensitive,method,advantages,circumvents,shortcomings,manual,eration,development,provides,isothermal,automatic,during,epidemics
AB值：
0.515678
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