The outbreak of the CO VID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus,and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed.On the other hand,several concerning SARS-CoV-2 variants,including Omicron,are required to be identified as soon as the samples are identified as'positive'.Unfortunately,a traditional PCR test does not allow their specific identification.Herein,for the first time,we have developed MOPCS(Methodologies of Photonic CRISPR Sensing),which combines an optical sensing technology-surface plasmon resonance(SPR)with the'gene scissors'clustered regularly interspaced short palindromic repeat(CRISPR)technique to achieve both high sensitivity and specificity when it comes to measurement of viral variants.MOPCS is a low-cost,CRISPR/Cas12a-system-empowered SPR gene-detecting platform that can analyze viral RNA,without the need for amplification,within 38 min from sample input to results output,and achieve a limit of detection of 15 fM.MOPCS achieves a highly sensitive analysis of SARS-CoV-2,and mutations appear in variants B.1.617.2(Delta),B.1.1.529(Omicron)and BA.1(a subtype of Omicron).This platform was also used to analyze some recently collected patient samples from a local outbreak in China,identified by the Centers for Disease Control and Prevention.This innovative CRISPR-empowered SPR platform will further contribute to the fast,sensitive and accurate detection of target nucleic acid sequences with single-base mutations.

Zhi Chen;Jingfeng Li;Tianzhong Li;Taojian Fan;Changle Meng;Chaozhou Li;Jianlong Kang;Luxiao Chai;Yabin Hao;Yuxuan Tangl;Omar A.Al-Hartomy;Swelm Wageh;Abdullah G.Al-Sehemi;Zhiguang Luo;Jiangtian Yu;Yonghong Shao;Defa Li;Shuai Feng;William J.Liu;Yaqing He;Xiaopeng Ma;Zhongjian Xie;Han Zhang

Shenzhen Engineering Laboratory of Phosphorene and Optoelectronics;International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education;Shenzhen Institute of Translational Medicine;Department of Otolaryngology,Shenzhen Second People's Hospital;the First Affiliated Hospital;Institute of Microscale Optoelectronics,Shenzhen University,Shenzhen 518060,China;Shenzhen International Institute for Biomedical Research,Shenzhen 518116,China;Shenzhen Han's Tech Limited Company,Shenzhen 518000,China;Shenzhen Metasensing Tech Limited Company,Shenzhen 518000,China;Department of Physics,Faculty of Science,King Abdulaziz University,Jeddah 21589,Saudi Arabia;Research Center for Advanced Materials Science(RCAMS),King Khalid University,Abha 61413,Saudi Arabia;Department of Chemistry,College of Science,King Khalid University,Abha 61413,Saudi Arabia;Zhongmin(Shenzhen)Intelligent Ecology Co Ltd,Shenzhen 518055,China;Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,College of Physics and Optoelectronic Engineering,Shenzhen University,Shenzhen 518060,China;Department of Laboratory Medicine,Shenzhen Children's Hospital,Shenzhen 518038,China;Optoelectronics Research Center,School of Science,Minzu University of China,Beijing 100081,China;NHC Key Laboratory of Biosafety,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Research Unit of Adaptive Evolution and Control of Emerging Viruses,Chinese Academy of Medical Sciences,Beijing 102206,China;Institute of Pathogenic Organism,Shenzhen Center for Disease Control and Prevention,Shenzhen 518055,China;Respiratory Department,Shenzhen Children's Hospital,Shenzhen 518038,China;Institute of Pediatrics,Shenzhen Children's Hospital,Shenzhen 518038,China

国家科学评论（英文版）

[1]Zhi Chen;Jingfeng Li;Tianzhong Li;Taojian Fan;Changle Meng;Chaozhou Li;Jianlong Kang;Luxiao Chai;Yabin Hao;Yuxuan Tangl;Omar A.Al-Hartomy;Swelm Wageh;Abdullah G.Al-Sehemi;Zhiguang Luo;Jiangtian Yu;Yonghong Shao;Defa Li;Shuai Feng;William J.Liu;Yaqing He;Xiaopeng Ma;Zhongjian Xie;Han Zhang-.A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2)[J].国家科学评论（英文版）,2022(08):91-100

presymptomatic,MOPCS

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