典型文献
A novel strategy for high-specificity,high-sensitivity,and high-throughput study for gut microbiome metabolism of aromatic carboxylic acids
文献摘要：
Aromatic carboxylic acids(ACAs)may be as transformed key metabolites via gut microbiome for playing better pharmacological effects.However,it's rare to achieve high-specificity,high-sensitivity,and high-throughput detection simultaneously,especially,for tracing trace ACAs in gut microbiome.In this work,firstly,a novel dual-template and double-shelled molecularly imprinted 96-well microplates(DDMIPs)was designed and amplified signal for p-hydroxybenzoic acid(PBA)and 3,4,5-trimethoxycinnamic acid(TMA).Additionally,the DDMIPs and a stable isotope labeling derivatization(SILD)method combined with the ultra-high performance liquid chromatography triple quadrupole tandem mass spectrome-try(UHPLC-TQ MS)was firstly stepwise integrated,achieving high-effective,high-sensitive,and high-throughput study of gut microbiome metabolism.The whole strategy showed lower limits of detections(LODs)up to 1000 folds than the traditional method,and revealed a more real metabolism-time profile of PBA and TMA by 3-step signal amplification.The platform also laid the foundation for fast,simple,high-selective,high-effective,and high-throughput metabolism and pharmacological research.
文献关键词：
中图分类号：
作者姓名：
Ningning Zhao;Zhiqiang Liu;Junpeng Xing;Zhong Zheng;Fengrui Song;Shu Liu
作者机构：
National Center of Mass Spectrometry in Changchun and Jilin Provincial Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China;Institute of Applied Chemistry and Engineering,University of Science and Technology of China,Hefei 230029,China;State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China
文献出处：
引用格式：
[1]Ningning Zhao;Zhiqiang Liu;Junpeng Xing;Zhong Zheng;Fengrui Song;Shu Liu-.A novel strategy for high-specificity,high-sensitivity,and high-throughput study for gut microbiome metabolism of aromatic carboxylic acids)[J].中国化学快报（英文版）,2022(06):3031-3034
A类：
ACAs,DDMIPs,trimethoxycinnamic,SILD
B类：
novel,strategy,high,specificity,sensitivity,throughput,study,gut,microbiome,metabolism,aromatic,carboxylic,acids,Aromatic,may,transformed,key,metabolites,via,playing,better,pharmacological,effects,However,rare,achieve,simultaneously,especially,tracing,trace,In,this,work,firstly,dual,template,double,shelled,molecularly,imprinted,well,microplates,was,designed,amplified,signal,hydroxybenzoic,PBA,TMA,Additionally,stable,isotope,labeling,derivatization,method,combined,ultra,performance,liquid,chromatography,triple,quadrupole,tandem,mass,spectrome,try,UHPLC,TQ,stepwise,integrated,achieving,effective,sensitive,whole,showed,lower,limits,detections,LODs,folds,than,traditional,revealed,more,real,profile,by,amplification,platform,also,laid,foundation,fast,simple,selective,research
AB值：
0.59425
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