典型文献
A simple,rapid,and practical method for single-cell proteomics based on mass-adaptive coating of synthetic peptides
文献摘要：
Since a typical mammalian cell contains only 200-300 pico-grams of proteins and the lack of a proteome amplification strategy[1],a practical and high-throughput approach for single-cell pro-teomics has long been awaited.A series of methods have been explored to make single-cell proteomics available.The design of nanoPOTS and OAD chip-based processes minimizes sample prepa-ration steps and volumes to reduce sample loss[2,3].The team that developed nanoPOTS further combined the sample preparation with ultrasensitive liquid chromatography(LC)separations,high field asymmetric ion mobility spectrometry(FAIMS),and the match-between-runs(MBR)algorithm in MaxQuant to achieve 1475 proteins from single HeLa cells on the Thermo Orbitrap Eclipse mass spectrometry(MS)platform[4].The Slavov team established and developed a series of SCoPE-MS methods in 2018 and 2021,in which they used multiplex isobaric labels and carriers(hundreds of cells)to improve the acquisition throughputs and ion signals of single-cell peptides[5,6].The concept of multiplexing and carrier channels was soon adopted by others[7-9],and Schoof et al.[9]recently reported an average of 987 proteins from single cells.However,a new strategy is still required to overcome the lim-itations in experimental design,challenging sample preparation[2-4],and the adverse impact of carrier proteins on data quality[10].Here,we report a simple,rapid,and practical approach of mass-adaptive coating-assisted single-cell proteomics(Mad-CASP).Combined with the designed synthetic peptides and novel data acquisition strategy,1240±93(n=3)proteins were identified from a single HeLa cell by Mad-CASP.For rare clinical samples,Mad-CASP efficiently profiled the proteomic heterogeneity among 240 human CD34+single cells derived from chronic total occlusion(CTO)patients.
文献关键词：
中图分类号：
作者姓名：
Siqi Li;Kaichan Su;Zhenkun Zhuang;Qing Qin;Lei Gao;Yamei Deng;Xuyang Liu;Guixue Hou;Longtao Wang;Piliang Hao;Huanming Yang;Siqi Liu;Hongming Zhu;Yan Ren
作者机构：
BGI-Shenzhen,Shenzhen 518083,China;Department of Biology,University of Copenhagen,Copenhagen 2100,Denmark;BGI-Wuhan Clinical Laboratories,BGI-Shenzhen,Wuhan 430000,China;Department of Cardiology,Zhongshan Hospital,Fudan University,Shanghai Institute of Cardiovascular Disease,Shanghai 200032,China;Department of Cardiovascular Surgery,The First Affiliated Hospital of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China(USTC),Hefei 230001,China;School of Life Science and Technology,Shanghai Tech University,Shanghai 201210,China;Institute for Regenerative Medicine,Shanghai East Hospital,Tongji University School of Medicine,Shanghai 200120,China;Experiment Center for Science and Technology,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China
文献出处：
引用格式：
[1]Siqi Li;Kaichan Su;Zhenkun Zhuang;Qing Qin;Lei Gao;Yamei Deng;Xuyang Liu;Guixue Hou;Longtao Wang;Piliang Hao;Huanming Yang;Siqi Liu;Hongming Zhu;Yan Ren-.A simple,rapid,and practical method for single-cell proteomics based on mass-adaptive coating of synthetic peptides)[J].科学通报（英文版）,2022(06):581-584
A类：
teomics,nanoPOTS,Slavov,SCoPE,throughputs,Schoof,CD34+single
B类：
simple,rapid,practical,proteomics,mass,adaptive,coating,synthetic,peptides,Since,typical,mammalian,contains,only,pico,grams,proteins,lack,proteome,amplification,strategy,high,approach,has,long,been,awaited,series,methods,have,explored,make,available,OAD,chip,processes,minimizes,steps,volumes,reduce,loss,team,that,developed,further,combined,preparation,ultrasensitive,liquid,chromatography,LC,separations,field,asymmetric,mobility,spectrometry,FAIMS,match,between,runs,MBR,algorithm,MaxQuant,achieve,from,HeLa,cells,Thermo,Orbitrap,Eclipse,platform,established,which,they,used,isobaric,labels,carriers,hundreds,improve,acquisition,signals,concept,multiplexing,channels,was,soon,adopted,by,others,recently,reported,average,However,new,still,required,overcome,lim,itations,experimental,challenging,adverse,impact,data,quality,Here,assisted,Mad,CASP,Combined,designed,novel,were,identified,For,rare,clinical,samples,efficiently,profiled,heterogeneity,among,human,derived,chronic,total,occlusion,CTO,patients
AB值：
0.535238
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