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典型文献
Highly sensitive smartphone-based detection of Listeria monocytogenes using SYTO9
文献摘要:
Listeriosis is caused by Listeria monocytogenes(LM)and is currently considered to be one of the leading food-borne diseases worldwide,with mortality rate of 20%~30%.Currently,detection methods for LM are time-consuming with low sensitivity,and delayed detection results.SYTO9 has a high affinity for DNA and exhibits enhanced fluorescence upon binding.Therefore,this study used SYTO9 staining and image processing to develop a rapid loop mediated isothermal amplification(LAMP)detection method for LM.Smartphone was successfully used for detecting the color change in different concentrations of LM.Besides,the optimized LAMP reaction temperature was 63℃by color identification,and the limit of detection for LM was 6 copies/μL in the green channel.So,the developed method,based on image processing,is simple,sensitive and rapid,which provides a new idea and method for rapid detection of LM and other food-borne bacterial pathogens.
文献关键词:
作者姓名:
Sha Liu;Xuliang He;Tao Zhang;Kaixuan Zhao;Changhu Xiao;Zengrui Tong;Lian Jin;Nongyue He;Yan Deng;Song Li;Yuan Guo;Zhu Chen
作者机构:
Hunan Key Laboratory of Biomedical Nanomaterials and Devices,Hunan University of Technology,Zhuzhou 412007,China;Department of General Surgery,People's Hospital of Zhuzhou City Affiliated to Changsha Medical College,Zhuzhou 412011,China;State Key Laboratory of Bioelectronics,School of Biological and Medical Engineering,Southeast University,Nanjing 210096,China;National&Local Joint Engineering Research Center for Research and Technology of Advanced Packaging Materials,Zhuzhou 412007,China;Department of Laboratory,Central Hospital of Zhuzhou City,Zhuzhou 412007,China
引用格式:
[1]Sha Liu;Xuliang He;Tao Zhang;Kaixuan Zhao;Changhu Xiao;Zengrui Tong;Lian Jin;Nongyue He;Yan Deng;Song Li;Yuan Guo;Zhu Chen-.Highly sensitive smartphone-based detection of Listeria monocytogenes using SYTO9)[J].中国化学快报(英文版),2022(04):1933-1935
A类:
SYTO9,Listeriosis,Smartphone
B类:
Highly,sensitive,smartphone,detection,Listeria,monocytogenes,using,caused,by,LM,currently,considered,be,leading,food,borne,diseases,worldwide,mortality,rate,Currently,methods,are,consuming,low,sensitivity,delayed,results,has,high,affinity,exhibits,enhanced,fluorescence,upon,binding,Therefore,this,study,staining,image,processing,rapid,loop,mediated,isothermal,amplification,LAMP,was,successfully,detecting,color,change,different,concentrations,Besides,optimized,reaction,temperature,identification,limit,copies,green,channel,So,developed,simple,which,provides,new,idea,bacterial,pathogens
AB值:
0.544399
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