Near-infrared Ⅱ(NIR-Ⅱ)fluorescent nanoprobes hold great potential for biomedical applications.Elucidating the relationship between surface properties of NIR-Ⅱ nanoprobes and their biological behav-iors is particularly important for future probe design and their performance optimization.Despite the rapid development of NIR-Ⅱ nanoprobes,the distinct role of surface chirality on their biological fates has rarely been exploited.Herein,chiral NIR-Ⅱ fluorescent Ag2S quantum dots(QDs)are synthesized to investigate the relationship between their chirality and biological functions at both in vitro and in vivo levels.D-/L-Ag2S QDs exhibit significant differences on their interactions with serum proteins,which fur-ther affect the cellular uptake.As a result,D-Ag2S QDs can be internalized with higher efficiency(over 2-fold)than that of L-Ag2S QDs.Moreover,in vivo studies reveal that the chirality determines the primary localization of these chiral QDs,where a more efficient renal elimination of D-Ag2S QDs was observed than that of L-Ag2S QDs.Importantly,D-Ag2S QDs show preferential accumulation in tumor region than that of L-Ag2S QDs in orthotopic kidney tumor model,which points out a new avenue of enhancing tar-geting capabilities of nanoprobes by engineering their surface chirality.

Shaohua Qu;Qian Jia;Zheng Li;Zhongliang Wang;Li Shang

State Key Laboratory of Solidification Processing,School of Materials Science and Engineering,Northwestern Polytechnical University(NPU),Xi'an 710072,China;Lab of Molecular Imaging and Translational Medicine(MITM),Engineering Research Center of Molecular&Neuroimaging,Ministry of Education,School of Life Science and Technology,Xidian University,Xi'an 710126,China;Academy of Advanced Interdisciplinary Research,Xidian University,Xi'an 710071,China

科学通报（英文版）

[1]Shaohua Qu;Qian Jia;Zheng Li;Zhongliang Wang;Li Shang-.Chiral NIR-Ⅱ fluorescent Ag2S quantum dots with stereospecific biological interactions and tumor accumulation behaviors)[J].科学通报（英文版）,2022(12):1274-1283

stereospecific

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