典型文献
Fluorescent immunosensor based on fluorescence resonance energy transfer between CdSe/ZnS quantum dots and Au nanorods for PRRSV detection
文献摘要：
An ultrasensitive and selective sandwich-type fluorescent immunosensor based on fluorescence resonance energy transfer(FRET)between CdSe/ZnS quantum dots(QDs)and Au nanorods(AuNRs)was developed for the rapid detection of porcine reproductive and respiratory syndrome virus(PRRSV).Modified CdSe/ZnS QDs and AuNRs were bioconjugated with primary antibodies M and GP5,respectively.When the target antigen was present,a well-known sandwich-type form CdSe/ZnS QDs-M antibody-antigen-GP5 antibody-AuNRs was produced,and thus reducing the distance between the QDs and NRs.As a result,FRET occurred and the fluorescence intensity of CdSe/ZnS QDs decreased,and this decrease was used to determine the antigen concentration.Ultrasensitive detection of PRRSV at low concentrations in swine serum was achieved with a limit of detection of 0.55 TCID50/mL and a linear detection range of 101-3.5 x 104 TCID5o/mL.Therefore,the fluorescent immunosensor is se-lective and highly sensitive;specifically,it can detect PRRSV at low concentrations in swine serum over a large concentration range.This proposed detection method can facilitate the development of high-performance fluo-rescent immunosensors for the detection of PRRSV and other antigens.
文献关键词：
中图分类号：
作者姓名：
Xue Li;Jinhua Li;Wentao Yang;Dandan Han;Na Yao;Hongbin Zhao;Xueying Chu;Xuemei Liang;Chunguang Bi;Chunfeng Wang;Guilian Yang
作者机构：
College of Information Technology,Jilin Agricultural University,Changchun,130118,China;Nanophotonics and Biophotonics Key Laboratory of Jilin Province,Changchun University of Science and Technology,Changchun,130022,China;Jilin Provincial Engineering Research Center of Animal Probiotics,Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding,Jilin Agricultural University,Changchun,130118,China;Changchun Huili Biotech Co.,Ltd.,Changchun,130102,China;Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development,College of Life Sciences,Jilin Agricultural University,Changchun,130118,China;State Key Laboratory of Advanced Materials for Smart Sensing,General Research Institute for Nonferrous Metals,Beijing,100088,China
文献出处：
引用格式：
[1]Xue Li;Jinhua Li;Wentao Yang;Dandan Han;Na Yao;Hongbin Zhao;Xueying Chu;Xuemei Liang;Chunguang Bi;Chunfeng Wang;Guilian Yang-.Fluorescent immunosensor based on fluorescence resonance energy transfer between CdSe/ZnS quantum dots and Au nanorods for PRRSV detection)[J].自然科学进展·国际材料（英文）,2022(02):157-162
A类：
bioconjugated,TCID5o
B类：
Fluorescent,fluorescence,resonance,energy,transfer,between,CdSe,ZnS,quantum,dots,nanorods,PRRSV,detection,An,ultrasensitive,selective,sandwich,type,fluorescent,FRET,QDs,AuNRs,was,developed,rapid,porcine,reproductive,respiratory,syndrome,virus,Modified,were,primary,antibodies,GP5,respectively,When,target,present,well,known,antibody,produced,thus,reducing,distance,result,occurred,intensity,decreased,this,used,determine,Ultrasensitive,low,concentrations,swine,serum,achieved,limit,TCID50,linear,range,Therefore,highly,specifically,can,over,large,This,proposed,method,facilitate,development,performance,immunosensors,other,antigens
AB值：
0.425552
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