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典型文献
Advanced technologies for single-cell in situ protein profiling
文献摘要:
Cells are inherently heterogeneous to achieve a diverse spectrum of biological functions.To understand the underlying protein machinery that achieves these fascinating functions,it is important to develop advanced analytical methods that can profile proteins in their native environment,as protein expression,aggregation,degradation,and regulation define both normal phy-siology as well as pathogenicity.Genome and transcriptome sequencing have seen major advances at the single-cell levels,but comprehensive proteomic profiling is still challenging.The conventional proteomic methods,such as enzyme-linked im-munosorbent assay (ELISA),western blot,and protein chips,can characterize biomarkers of interest.Still,these ensemble techniques are unsuitable for single-cell studies.Increasing evidence has shown the significance of in situ,sensitive,quantitative,and multiplexed profiling of biomarkers in single cells for diagnosis and treatment guidance.Here,we review the recent development of advanced imaging and spectroscopy techniques,including mass cytometry,immunofluorescence,and surface-enhanced Raman spectrometry (SERS) for single-cell proteomic imaging.We also provide our view on the challenges and the outlook.
文献关键词:
作者姓名:
Wen Zhou;Xiaoyue Ni;Chen Xie;Quli Fan;Dingbin Liu
作者机构:
State Key Laboratory of Organic Electronics and Information Displays,and Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM),Institute of Advanced Materials (IAM),Nanjing University of Posts & Telecommunications,Nanjing 210023,China;State Key Laboratory of Medicinal Chemical Biology,Research Center for Analytical Sciences,Tianjin Key Laboratory of Biosensing and Molecular Recognition,College of Chemistry,Nankai University,Tianjin 300071,China
引用格式:
[1]Wen Zhou;Xiaoyue Ni;Chen Xie;Quli Fan;Dingbin Liu-.Advanced technologies for single-cell in situ protein profiling)[J].中国科学:化学(英文版),2022(01):48-67
A类:
siology
B类:
Advanced,technologies,single,situ,profiling,Cells,are,inherently,heterogeneous,diverse,spectrum,biological,functions,To,understand,underlying,machinery,that,achieves,these,fascinating,important,advanced,analytical,methods,profile,proteins,their,native,environment,expression,aggregation,degradation,regulation,define,both,normal,phy,well,pathogenicity,Genome,transcriptome,sequencing,have,seen,major,advances,levels,but,comprehensive,proteomic,still,challenging,conventional,such,enzyme,linked,munosorbent,assay,western,blot,chips,characterize,biomarkers,interest,Still,ensemble,techniques,unsuitable,studies,Increasing,evidence,has,shown,significance,sensitive,quantitative,multiplexed,cells,diagnosis,treatment,guidance,Here,review,recent,development,imaging,spectroscopy,including,mass,cytometry,immunofluorescence,surface,enhanced,Raman,spectrometry,SERS,We,also,provide,our,challenges,outlook
AB值:
0.667822
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