A simple and efficient dithizone-functionalized solid-phase extraction(SPE)procedure,on-line coupled with high-performance liquid chromatography(HPLC)-inductively coupled plasma mass spectrometry,was developed for the first time for enrichment and determi-nation of ultra-trace mercury(Hg)species(inorganic divalent Hg(Hg(I)),methylmercury(CH3Hg(I))and ethylmercury(C2H5Hg(Ⅱ))in cereals and environmental samples.In the pro-posed method,functionalization of the commercial C18 column with dithizone,enrichment,and elution of the above Hg species can be completed online with the developed SPE device.A simple solution of 2-mercaptoethanol(1％(V/V))could be used as an eluent for both the SPE and HPLC separation of Hg species,significantly simplifying the method and instrumen-tation.The online SPE method was optimized by varying dithizone dose,2-mercaptoethanol concentration,and sample volume.In addition,the effect of pH,coexisting interfering ions,and salt effect on the enrichment was also discussed.Under the optimized conditions,the detection limits of Hg species for 5 mL water sample were 0.15 ng/L for Hg(Ⅱ),0.07 ng/L for CH3Hg(Ⅱ),and 0.04 ng/L for C2H5Hg(Ⅱ)with recoveries in the range of 85％-100％.The developed dithizone-functionalized C18 SPE column can be reused after a single function-alization,which significantly simplifies the enrichment step.Moreover,the stability of Hg species enriched on the SPE column demonstrated its suitability for field sampling of Hg species for later laboratory analysis.This environment-friendly method offers a robust tool to detect ultra-trace Hg species in cereals and environmental samples.

Ying Wang;Ailing Zhu;Yingying Fang;Changjun Fan;Yanli Guo;Zhiqiang Tan;Yongguang Yin;Yong Cai;Guibin Jiang

Laboratory of Environmental Nanotechnology and Health Effect,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China;State Key Laboratory of Environmental Chemistry and Ecotoxicology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China;University of Chinese Academy of Sciences,Beijing 100049,China;School of Environment,Hangzhou Institute for Advanced Study,UCAS,Hangzhou 310024,China;Shimadzu China Innovation Center,Shimadzu(China)Co.LTD,Beijing 100020,China;Department of Chemistry and Biochemistry,Florida International University,Miami,FL 33199,United States

环境科学学报（英文版）

[1]Ying Wang;Ailing Zhu;Yingying Fang;Changjun Fan;Yanli Guo;Zhiqiang Tan;Yongguang Yin;Yong Cai;Guibin Jiang-.Dithizone-functionalized C18 online solid-phase extraction-HPLC-ICP-MS for speciation of ultra-trace organic and inorganic mercury in cereals and environmental samples)[J].环境科学学报（英文版）,2022(05):403-410

Dithizone,dithizone,CH3Hg,ethylmercury,C2H5Hg,mercaptoethanol,eluent,instrumen

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