The development of reactive oxygen species(ROS)generation agents that can selectively produce sufficient ROS at the tumor site without external energy stimulation is of great significance for the further clinical application of ROS-based therapies.Herein,we designed a cascade-responsive ROS nanobomb(ZnO2@Ce6/CaP@CPPO/BSA,designated as Z@Ce6/CaP@CB)with domino effect and without external stimulation for the specific generation of multiple powerful ROS storms at the tumor site.The calcium phosphate shell and ZnO2 core gradually degrade and release Ca2+,Zn2+and hydrogen peroxide(H2O2)under acid stimulation.On the one hand,Zn2+can enhance the generation of endogenous superoxide anions(·O2-)and H2O2 through the inhibition of the mitochondrial electron transport chain.On the other hand,the generation of large amounts of exogenous H2O2 can cause oxidative damage to tumor cells and further activate bis[2,4,5-trichloro-6-(pentyloxycarbonyl)phenyl]oxalate(CPPO)-mediated chemiexcited photodynamic therapy.In addition,the oxidative stress caused by the generated ROS can lead to the uncontrolled accumulation of Ca2+in cells and further result in Ca2+overload-induced cell death.Therefore,the introduction of Z@Ce6/CaP@CB nanobombs triggered the'domino effect'that caused multiple heavy ROS storms and Ca2+overload in tumors and effectively activated anti-tumor immune response.

State Key Laboratory of Rare Earth Resource Utilization,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China;School of Applied Chemistry and Engineering,University of Science and Technology of China,Hefei 230026,China;Department of Chemistry,Tsinghua University,Beijing 100084,China

[1]Yang Liu;Yinghui Wang;Shuyan Song;Hongjie Zhang-.Cascade-responsive nanobomb with domino effect for anti-tumor synergistic therapies)[J].国家科学评论（英文版）,2022(03):28-37

nanobomb,ZnO2,CPPO,Zn2+can,pentyloxycarbonyl,chemiexcited,Ca2+overload,nanobombs

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