Currently,artificial enzymes-based photodynamic therapy(PDT)is attractive due to its efficient capacity to change the immunosuppressive tumor microenvironment(TME).It is of great significance to study the therapeutic mechanism of novel artificial enzymes in TME through a monitoring strategy and improve the therapeutic effect.In this study,Au@carbon dots(Au@CDs)nanohybrids with a core-shell structure are synthesized,which not only exhibit tunable enzyme-mimicking activity under near-infrared(NIR)light,but also excellent surface-enhanced Raman scattering(SERS)properties.Therefore,Au@CDs show a good capability for monitoring NIR-photoinduced peroxidase-like catalytic processes via a SERS strategy in tumor.Moreover,the Au@CDs deplete glutathione with the cascade catalyzed reactions,thus elevating intratumor oxidative stress amplifying the reactive oxygen species damage based on the NIR-photoinduced enhanced peroxidase and glutathione oxidase-like activities,showing excellent and fast PDT therapeutic effect promoted by photothermal property in 3 min,finally leading to apoptosis in cancer cells.Through SERS monitoring,it is further found that after removing the NIR light source for 33 min,the reactive oxygen species(ROS)activity of the TME is counteracted and eliminated due to the presence of glutathione.This work presents a guidance to rationally design of artificial enzyme for ROS-involved therapeutic strategies and a new spectroscopic tool to evaluate the tumor catalytic therapy.

Linjia Li;Jin Yang;Jiahui Wei;Chunhuan Jiang;Zhuo Liu;Bai Yang;Bing Zhao;Wei Song

State Key Laboratory of Supramolecular Structure and Materials,College of Chemistry,Jilin University,Changchun 130012,China;Department of Vascular Surgery of China-Japan Union Hospital,Jilin University,Changchun 130031,China;College of Basic Medical Sciences,Jilin University,Changchun 130021,China;Department of Laboratory Animals,College of Animal Sciences,Jilin University,Changchun 130062,China;State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China

光：科学与应用（英文版）

[1]Linjia Li;Jin Yang;Jiahui Wei;Chunhuan Jiang;Zhuo Liu;Bai Yang;Bing Zhao;Wei Song-.SERS monitoring of photoinduced-enhanced oxidative stress amplifier on Au@carbon dots for tumor catalytic therapy)[J].光：科学与应用（英文版）,2022(10):2492-2502

intratumor

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