典型文献
Enzyme-catalyzed deposition of polydopamine for amplifying the signal inhibition to a novel Prussian blue-nanocomposite and ultrasensitive electrochemical immunosensing
文献摘要：
The uncontrollable synthesis of Prussian blue(PB)and its weak stability toward OH-are great challenges affecting its electrochemical biosensing application.Herein we utilize the unique properties of chitosan(CS)to realize the facile and controllable synthesis of a CS-PB nanocomposite and combine it with the urease-catalyzed deposition of polydopamine(PDA)for amplifying the electrochemical signal inhibition of PB to develop a novel immunosensing method for protein detection.The immunosensor was constructed on a CS-PB modified electrode,and a urease-functionalized silica nanoprobe was prepared for tracing its sandwich immunoassay toward the model analyte of carcinoembryonic antigen.Besides the electrochem-ical impedance effect of the quantitatively captured nanoprobes,their enzymatic reaction can release numerous OH-to destroy the PB crystals and also induce the PDA deposition onto the immunosensor.These caused drastic electrochemical signal inhibition to PB.Based on the above multi-signal amplifica-tion mechanism,the method exhibits a very low detection limit of 0.042 pg mL-1 along with a very wide linear range of six-order of magnitude.In addition,the CS-PB based immunosensor has excellent speci-ficity,repeatability,stability and reliability.Thus this PB nanocomposite and the proposed electrochemical immunosensing method reveal a promising potential for future applications.
文献关键词：
中图分类号：
作者姓名：
Wan Huang;Peng Guo;Bo Li;Li Fu;Cheng-Te Lin;Aimin Yu;Guosong Lai
作者机构：
Hubei Key Laboratory of Pollutant Analysis&Reuse Technology,College of Chemistry and Chemical Engineering,Hubei Normal University,Huangshi 435002,China;College of Materials and Environmental Engineering,Hangzhou Dianzi University,Hangzhou 310018,China;Ningbo Institute of Materials Technology and Engineering,Chinese Academy of Sciences,Ningbo 315201,China;Department of Chemistry and Biotechnology,Faculty of Science,Engineering and Technology,Swinburne University of Technology,Hawthorn,VIC 3122,Australia
文献出处：
引用格式：
[1]Wan Huang;Peng Guo;Bo Li;Li Fu;Cheng-Te Lin;Aimin Yu;Guosong Lai-.Enzyme-catalyzed deposition of polydopamine for amplifying the signal inhibition to a novel Prussian blue-nanocomposite and ultrasensitive electrochemical immunosensing)[J].材料科学技术（英文版）,2022(07):166-173
A类：
immunosensing,amplifica
B类：
Enzyme,catalyzed,deposition,polydopamine,amplifying,signal,inhibition,novel,Prussian,blue,nanocomposite,ultrasensitive,electrochemical,uncontrollable,synthesis,PB,weak,stability,toward,great,challenges,affecting,biosensing,Herein,utilize,unique,properties,chitosan,CS,realize,facile,combine,urease,PDA,develop,method,protein,detection,immunosensor,was,constructed,modified,electrode,functionalized,silica,prepared,tracing,sandwich,immunoassay,model,analyte,carcinoembryonic,antigen,Besides,impedance,effect,quantitatively,captured,nanoprobes,their,enzymatic,reaction,can,release,numerous,destroy,crystals,also,induce,onto,These,caused,drastic,Based,above,multi,mechanism,exhibits,very,low,limit,pg,along,wide,linear,range,six,order,magnitude,In,addition,has,excellent,speci,ficity,repeatability,reliability,Thus,this,proposed,reveal,promising,potential,future,applications
AB值：
0.555
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