典型文献
Ultrasensitive detection of IgE levels based on magnetic nanocapturer linked immunosensor assay for early diagnosis of cancer
文献摘要：
Rapid and accurate detection of immunoglobulin E(IgE)in serum and reduction of serum dosage are of great significance for clinical detection.Herein,we described a rapid magnetic separation of IgE from pa-tient serum based on Fe304@SiO2-NTA@026 sdab as the capture probe and multiple horseradish peroxi-dase(HRP)-labeled antibodies linked gold nanoparticles(AuNPs)as chemiluminescence(CL)signal ampli-fier for ultrasensitive detection of total IgE.Results showed that the limit of detection of our immunosen-sor system in serum samples was 0.03 kU/L,which is lowest in comparison with current methods,and far lower than that of ImmunoCAP for IgE detection(0.1 kU/L).Furthermore,our immunosensor pos-sessed satisfied repeatability and accuracy,as well as good stability.In comparison with the ImmunoCAP for the quantitative detection of IgE,highly consistent results were achieved in 20 serum samples.Spe-cially,this method was also successfully utilized for assessing the IgE traces in breast cancer patients,which provides a new idea for the diagnosis of early cancer.Therefore,we believe that such versatile immunosensor will offer an alternative method for the on-site monitoring and determination of various IgE-related diseases.
文献关键词：
中图分类号：
作者姓名：
Yongxin Liu;Mengda Cao;Zhongxi Huang;Changmin Yu;Naidi Yang;Qiong Wu;Liang Shi;Wenjie Duan;Yi Zhu;Jifu Wei;Lin Li;Wei Huang
作者机构：
Key Laboratory of Flexible Electronics(KLOFE)&Institute of Advanced Materials(IAM),Nanjing Tech University(NanjingTech),Nanjing 211816,China;Department of Pharmacy,Jiangsu Institute of Cancer Research&the Affiliated Cancer Hospital of Nanjing Medical University,Nanjing 210009,China;Department of Pharmacy,Zhongda Hospital,School of Medicine,Southeast University,Nanjing 210044,China;Research Division of Clinical Pharmacology&Clinical Allergy Center,the First Affiliated Hospital of Nanjing Medical University;Shaanxi Institute of Flexible Electronics(SIFE),Northwestern Polytechnical University(NPU),Xi'an 710072,China;State Key Laboratory of Coordination Chemistry,Nanjing University,Nanjing 210023,China
文献出处：
引用格式：
[1]Yongxin Liu;Mengda Cao;Zhongxi Huang;Changmin Yu;Naidi Yang;Qiong Wu;Liang Shi;Wenjie Duan;Yi Zhu;Jifu Wei;Lin Li;Wei Huang-.Ultrasensitive detection of IgE levels based on magnetic nanocapturer linked immunosensor assay for early diagnosis of cancer)[J].中国化学快报（英文版）,2022(04):1855-1860
A类：
nanocapturer,sdab,immunosen
B类：
Ultrasensitive,detection,IgE,levels,magnetic,linked,immunosensor,assay,early,diagnosis,cancer,Rapid,accurate,immunoglobulin,serum,reduction,dosage,are,great,significance,clinical,Herein,described,rapid,separation,from,Fe304,SiO2,NTA,probe,multiple,horseradish,peroxi,dase,HRP,labeled,antibodies,gold,nanoparticles,AuNPs,chemiluminescence,CL,signal,ampli,fier,ultrasensitive,total,Results,showed,that,limit,our,system,samples,was,kU,which,lowest,comparison,current,methods,far,lower,than,ImmunoCAP,Furthermore,pos,sessed,satisfied,repeatability,accuracy,well,good,stability,In,quantitative,highly,consistent,results,were,achieved,Spe,cially,this,also,successfully,utilized,assessing,traces,breast,patients,provides,new,idea,Therefore,believe,such,versatile,will,offer,alternative,site,monitoring,determination,various,related,diseases
AB值：
0.608255
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