典型文献
Simultaneous determination of LY3214996,abemaciclib,and M2 and M20 metabolites in human plasma,cerebrospinal fluid,and brain tumor by LC-MS/MS
文献摘要：
A sensitive and rapid liquid chromatography tandem mass spectrometry(LC-MS/MS)method was established for the quantification of total and unbound concentrations of LY3214996,an extracellular signal-regulated kinase inhibitor;abemaciclib,a cyclin-dependent kinase 4/6 inhibitor;and abemaciclib active metabolites,M2 and M20,in human plasma,brain tumor,and cerebrospinal fluid samples.The method was validated over a concentration range of 0.2-500 nM within a total run time of 3.8 min using isocratic elution on a Kinetex? Fs column.Detection was performed on a Sciex QTRAP 6500+mass spectrometer employing multiple reaction monitoring mode under positive electrospray ionization.The intra-and inter-batch accuracy as well as the precision of the method for all matrices was within±20％and≤20％at the lower limit of quantification,and within±15％and≤15％for other quality control levels for all analytes.The unbound fractions of drugs and metabolites in spiked and patient samples were determined using an optimized equilibrium dialysis.The validated method was successfully applied in a phase 0/2 clinical trial to assess the central nervous system penetration of LY3214996 and abemaciclib.
文献关键词：
中图分类号：
作者姓名：
Tigran Margaryan;Mackenna Elliott;Nader Sanai;Artak Tovmasyan
作者机构：
Ivy Brain Tumor Center,Barrow Neurological Institute,Phoenix,AZ,85013,USA
文献出处：
引用格式：
[1]Tigran Margaryan;Mackenna Elliott;Nader Sanai;Artak Tovmasyan-.Simultaneous determination of LY3214996,abemaciclib,and M2 and M20 metabolites in human plasma,cerebrospinal fluid,and brain tumor by LC-MS/MS)[J].药物分析学报（英文）,2022(04):601-609
A类：
LY3214996,abemaciclib,isocratic,Sciex,6500+mass
B类：
Simultaneous,determination,M20,metabolites,human,plasma,cerebrospinal,fluid,brain,tumor,by,LC,sensitive,rapid,liquid,chromatography,tandem,spectrometry,method,was,established,quantification,total,unbound,concentrations,extracellular,signal,regulated,kinase,inhibitor,cyclin,dependent,active,samples,validated,over,range,nM,within,run,using,elution,Kinetex,Fs,column,Detection,performed,QTRAP,spectrometer,employing,multiple,reaction,monitoring,mode,under,positive,electrospray,ionization,intra,inter,batch,accuracy,well,precision,all,matrices,lower,limit,other,quality,control,levels,analytes,fractions,drugs,spiked,patient,were,determined,optimized,equilibrium,dialysis,successfully,applied,phase,clinical,trial,assess,central,nervous,system,penetration
AB值：
0.551723
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