典型文献
Simultaneous determination of indole metabolites of tryptophan in rat feces by chemical labeling assisted liquid chromatography-tandem mass spectrometry
文献摘要：
As the connecting part of diet and host physiology,intestinal microbes can convert the ingested diet into a huge number of physiologically active small molecules.Indole metabolites of tryptophan are pre-cursors or signal molecules for many biologically active substances,which are involved in serotonin and microbial catabolism pathways.To understand the influence of tryptophan metabolism in the in-testinal environment on the neurological and immune systems at the molecular level,it is important to establish a high-coverage analytical method to comprehensively analyze the metabolites involved in tryptophan metabolism.However,due to a small molecular weight and poor response during mass spectrometry analysis,as well as weak retention on the reversed-phase chromatography,determina-tion of indole metabolites of tryptophan is challenging.Here,we proposed a method for the simulta-neous determination of 20 indole metabolites of tryptophan in a single run on reversed-phase chro-matography by chemical labeling coupled to liquid chromatography-tandem mass spectrometry analy-sis.4-(Dimethylamino)benzaldehyde(DMAB)was used for the labeling of indole metabolites of trypto-phan,which could significantly improve the detection sensitivities and retention of these metabolites on reversed-phase chromatography.With the developed method,we realized the sensitive detection and comprehensive analysis of 15 endogenous indole metabolites of tryptophan in rat feces samples with functional dyspepsia intervention by acupuncture.The developed method offers a useful tool for study-ing tryptophan metabolism-related diseases.
文献关键词：
中图分类号：
作者姓名：
Qin-Feng Zhang;Hua-Ming Xiao;Jin-Tao Zhan;Bi-Feng Yuan;Yu-Qi Feng
作者机构：
Department of Chemistry,Wuhan University,Wuhan 430072,China;School of Public Health,Wuhan University,Wuhan 430071,China
文献出处：
引用格式：
[1]Qin-Feng Zhang;Hua-Ming Xiao;Jin-Tao Zhan;Bi-Feng Yuan;Yu-Qi Feng-.Simultaneous determination of indole metabolites of tryptophan in rat feces by chemical labeling assisted liquid chromatography-tandem mass spectrometry)[J].中国化学快报（英文版）,2022(11):4746-4749
A类：
Dimethylamino,trypto,phan
B类：
Simultaneous,determination,indole,metabolites,tryptophan,rat,feces,by,chemical,labeling,assisted,liquid,chromatography,tandem,mass,spectrometry,connecting,part,diet,host,physiology,intestinal,microbes,convert,ingested,into,huge,number,physiologically,active,small,molecules,Indole,are,cursors,signal,many,biologically,substances,which,involved,serotonin,microbial,catabolism,pathways,To,understand,influence,metabolism,environment,neurological,immune,systems,molecular,level,important,establish,high,coverage,analytical,method,comprehensively,analyze,However,due,weight,poor,response,during,analysis,well,weak,retention,reversed,phase,challenging,Here,proposed,simulta,single,run,coupled,benzaldehyde,DMAB,was,used,could,significantly,improve,detection,sensitivities,these,With,developed,realized,sensitive,endogenous,samples,functional,dyspepsia,intervention,acupuncture,offers,useful,tool,study,related,diseases
AB值：
0.500017
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
