Objective:Numerous studies have demonstrated the close relationship between chronic stress and blood pressure (BP).Hypertensive subjects exhibit exaggerated reactions to stress,especially higher BP.The mechanisms by which stress affects pre-existing hypertension still need to be explored.Danzhi Xiaoyao Powder (DP),a historical traditional Chinese medicine formula,is a promising treatment for BP control in hypertensive patients under stress.The present study investigated the metabolomic disrup-tion caused by chronic stress and the treatment effect and mechanism of DP.Methods:Spontaneously hypertensive rats (SHRs) were subjected to chronic restraint stress (CRS) for 4 weeks.BP was measured via the tail-cuff method,and anxiety-like behavior was quantified using the elevated-plus-maze test.Meanwhile,DP was administered intragastrically,and its effects were observed.Global metabolomic analysis was performed using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry,followed by multivariate statistical anal-ysis to detect differential metabolites and pathways.Results:DP alleviated the CRS-induced increase in BP and anxiety-like behavior.Systematic metabolic differences were found among the three study groups.A total of 29 differential plasma metabolites were identified in both positive-and negative-ion modes.These metabolites were involved in triglyceride metabolism,amino acid (phenylalanine,tryptophan,and glycine) metabolism,and steroid hormone pathways.Conclusion:These findings expose the metabolomic disturbances induced by chronic stress in SHRs and suggest an innovative treatment for this disorder.

Shuai Chen;Jin Hu;Deng-cheng Lu;Hong-yi Liu;Shan-shan Wei

College of Basic Medical Sciences,Yunnan University of Chinese Medicine,Kunming 650500,Yunnan Province,China;Department of Endocrinology,Yunnan Provincial Hospital of Traditional Chinese Medicine,Kunming 650021,Yunnan Province,China;Key Laboratory of Syndrome Micro-Differentiation of Yunnan Province,Kunming 650500,Yunnan Province,China

结合医学学报（英文版）

[1]Shuai Chen;Jin Hu;Deng-cheng Lu;Hong-yi Liu;Shan-shan Wei-.Metabolomic characteristics of spontaneously hypertensive rats under chronic stress and the treatment effect of Danzhi Xiaoyao Powder,a traditional Chinese medicine formula)[J].结合医学学报（英文版）,2022(01):73-82

Danzhi,disrup

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