The fruit of Ziziphus jujuba Mill., known as Hongzao (or Hong-Zao) in Chinese and cultivated in China for more than 4000 years, has shown to have hepatoprotective property. In previous study, we have isolated and identified 27 known compounds from Z. jujuba fruits, which demonstrated anti-tumor activity. In this study, a high-performance liquid chromatography-diode-array detection-mass spectrometry (HPLC-DAD-MS) method was successfully applied to the simultaneous characterization and quantitation of 18 constituents in 28 Z. jujuba samples, comprised of 12 cultivars from different regions in China, by comparing their HPLC retention times, MS spectra, UV spectra, and NMR data with those of reference compounds. The quantitative method was validated with excellent linearity (R2 > 0.9991), preferable intra- and inter-day precisions (RSD < 2.78％), and good recoveries (94.96％–102.65％). The content variation of 18 compounds was analyzed by a chemometric method (hierarchical cluster analysis). In addition, these constituents showed protection against carbon tetrachloride (CCl4) intoxicated HepG2 cell lines by decreasing lactic dehydrogenase (LDH) levels. Results in this study illustrated that the content of all 18 compounds examined has significant difference and variation among cultivars and extracts. The proposed method can serve as a prerequisite for quality control of bioactive compounds in Z. jujuba products.

Tianyi Wang;Sen Guo;Xiameng Ren;Junfeng Du;Lu Bai;Xueqin Cui;Chi-Tang Ho;Naisheng Bai

College of Food Science and Technology,Northwest University,Xi'an 710069,China;College of Chemical Engineering,Northwest University,229 Taibai North Road,Xi'an 710069,China;Shaanxi Family Forestry Bureau,Shaanxi Jiaxian Development and Reform and Science and Technology Bureau,Yulin 719200,China;Department of Food Science,Rutgers University,New Brunswick 08901,USA

食品科学与人类健康（英文）

[1]Tianyi Wang;Sen Guo;Xiameng Ren;Junfeng Du;Lu Bai;Xueqin Cui;Chi-Tang Ho;Naisheng Bai-.Simultaneous quantification of 18 bioactive constituents in Ziziphus jujuba fruits by HPLC coupled with a chemometric method)[J].食品科学与人类健康（英文）,2022(04):771-780

chemometric,Hongzao

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