Objective:Corydalis bungeana(CB)is a well-used medicinal herb in Mongolian folk medicine and has been traditionally applied as an antiobesity agent.However,the evidence-based pharmacological effects of CB and its specific metabolic alterations in the obese model are not entirely understood.This study aimed to utilize untargeted metabolomic techniques to identify biomarkers and gain mechanistic insight into the serum metabolite alterations associated with weight loss and lipid metabolism in obese rats.Methods:A high-fat high-sugar(HFHS)diet was used to induce obese models in rats.CB extract was orally gavaged at 0.18,0.9 and 1.8 g/kg doses for six weeks,and feed intake,body weight,fat pad weight,and blood indexes were measured.Blood serum metabolites were evaluated by gas chromatography/quadrupole time-of-flight tandem mass spectrometry(GC-TOF/MS).Results:The results showed that compared with the obese group,the administration of CB extract caused significant decreases in body weight(P＜0.05),feed intake,Lee's index,and perirenal,mesenteric,epi-didymal fat weight.CB extract also reduced blood triglyceride and total cholesterol levels(P＜0.05)of obese rats.Metabolomic findings showed that nine differential metabolites,including pyruvic acid,D-glucuronic acid,malic acid,dimethylglycine,oxoglutaric acid,pantothenic acid,sorbitol acid,fumaric acid and glucose 6-phosphate were identified under CB treatment and altered metabolic pathways such as TCA cycle,pantothenate and CoA biosynthesis,and glycolysis/gluconeogenesis.Conclusion:This study demonstrated weight loss and lipid lowering effects of CB on HFHS diet-induced obese rats and identified nine metabolites as potential biomarkers for evaluating the favorable therapeu-tic mechanism of CB via regulation of lipid and glucose metabolism.

Minghai Fu;Terigele Bao;Hongzhen Yu;LiSha A.;HuiFang Li;Genna Ba;Sungbo Cho

Key Laboratory of Tropical Translational Medicine of Ministry of Education,Hainan Key Laboratory for Research and Development of Tropical Herbs,School of Pharmacy,Hainan Medical University,Haikou 571199,China;NMPA Key Laboratory of Quality Control of Traditional Chinese Medicine(Mongolian Medicine),Inner Mongolia Minzu University,Tongliao 028000,China

中草药（英文版）

[1]Minghai Fu;Terigele Bao;Hongzhen Yu;LiSha A.;HuiFang Li;Genna Ba;Sungbo Cho-.Metabolomics investigation on antiobesity effects of Corydalis bungeana on high-fat high-sugar diet-induced obese rats)[J].中草药（英文版）,2022(03):414-421

HFHS,didymal,dimethylglycine,oxoglutaric,fumaric,pantothenate

Metabolomics,investigation,antiobesity,effects,Corydalis,bungeana,high,fat,sugar,diet,induced,obese,rats,Objective,CB,well,medicinal,herb,Mongolian,folk,medicine,has,been,traditionally,applied,agent,However,evidence,pharmacological,its,specific,metabolic,alterations,not,entirely,understood,This,study,aimed,utilize,untargeted,metabolomic,techniques,identify,biomarkers,gain,mechanistic,insight,into,serum,associated,weight,loss,lipid,metabolism,Methods,was,models,extract,orally,gavaged,doses,six,weeks,feed,intake,body,pad,blood,indexes,were,measured,Blood,metabolites,evaluated,by,gas,chromatography,quadrupole,flight,tandem,mass,spectrometry,TOF,Results,results,showed,that,compared,group,administration,caused,significant,decreases,Lee,perirenal,mesenteric,epi,also,reduced,triglyceride,total,cholesterol,levels,findings,nine,differential,including,pyruvic,acid,glucuronic,malic,pantothenic,sorbitol,glucose,phosphate,identified,treatment,altered,pathways,such,TCA,cycle,CoA,biosynthesis,glycolysis,gluconeogenesis,Conclusion,demonstrated,lowering,potential,evaluating,favorable,therapeu,mechanism,via,regulation

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