典型文献
Solid-phase alkylation:a low-loss and anti-interference sample preparation strategy for low-input proteome profiling
文献摘要：
Deep proteome profiling from trace biological samples are needed for important biomedical applications[1],but the sample losses from traditional sample preparation procedures are a major limitation[2].Therefore,considerable efforts have been made to improve it[3,4].Filter-aided sample preparation(FASP)developed by Mann's group[5]is recognized one of the most popular meth-ods,by which protein extraction,reduction,alkylation,and diges-tion are performed on a commercial spin filter.However,the strong detergents that are often utilized to aid cell disruption and hydrophobic protein solubilization are often incompatible with proteolysis and mass spectrometry(MS)analysis;they neces-sitate removal using multiple ultrafiltration operations,which not only increase sample handling time but also lead to sample loss.Recently,to improve proteome sample preparation,in-situ sample processing techniques such as nanodroplet processing platform(nanoPOTS)[6]have been developed to improve the efficiency and recovery of sample preparation through reducing the surface loss and enhancing the collision possibility of reactive molecules in a micro-scale confinement space,which enable the preparation of sub-microgram starting materials.
文献关键词：
中图分类号：
作者姓名：
Yilan Li;Huiming Yuan;Mengchun Cheng;Xudong Zhu;Kaiguang Yang;Weijie Zhang;Zhigang Sui;Chunyu Zhang;Lihua Zhang;Yukui Zhang
作者机构：
CAS Key Laboratory of Separation Science for Analytical Chemistry,National Chromatographic Research and Analysis Center,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China;University of Chinese Academy of Sciences,Beijing 100049,China;Medical Research Center,Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou 510120,China;The Second Affiliated Hospital of Dalian Medical University,Dalian 116021,China
文献出处：
引用格式：
[1]Yilan Li;Huiming Yuan;Mengchun Cheng;Xudong Zhu;Kaiguang Yang;Weijie Zhang;Zhigang Sui;Chunyu Zhang;Lihua Zhang;Yukui Zhang-.Solid-phase alkylation:a low-loss and anti-interference sample preparation strategy for low-input proteome profiling)[J].科学通报（英文版）,2022(16):1628-1631
A类：
FASP,sitate,nanodroplet,nanoPOTS,microgram
B类：
Solid,phase,alkylation,low,anti,interference,preparation,strategy,input,proteome,profiling,Deep,from,trace,biological,samples,are,needed,important,biomedical,applications,but,losses,traditional,procedures,major,limitation,Therefore,considerable,efforts,have,been,made,improve,Filter,aided,developed,by,Mann,group,recognized,one,most,popular,meth,ods,which,protein,extraction,reduction,diges,performed,commercial,spin,filter,However,strong,detergents,that,often,utilized,cell,disruption,hydrophobic,solubilization,incompatible,proteolysis,mass,spectrometry,analysis,they,neces,removal,using,multiple,ultrafiltration,operations,not,only,increase,handling,also,lead,Recently,situ,processing,techniques,such,platform,efficiency,recovery,through,reducing,surface,enhancing,collision,possibility,reactive,molecules,scale,confinement,space,enable,sub,starting,materials
AB值：
0.619394
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