典型文献
Rapid screening of SARS-CoV-2 inhibitors via ratiometric fluorescence of RBD—ACE2 complexes in living cells by competitive binding
文献摘要：
To the Editor:
The current coronavirus disease-19(COVID-19)pandemic spurs the development of antiviral drugs for SARS-CoV-2,as the number of patients with viral infections continues to rise globally in the context of widespread vaccination.Targeting the interaction between the receptor binding domain(RBD)of SARS-CoV-2 spike protein and the host cell ACE2 is a promising therapeutic strategy to effectively inhibit viral entry,because the strong binding between RBD and ACE2 is the first step of viral infec-tion1.Screening in live cells,represented by live or pseudo virus assays,have been approved to significantly improve effectiveness of the obtained inhibitors2,3.However,they are greatly limited by the biosafety level 3 facilities or multistep experimental proced-ures.Therefore,the development of rapid cell-based screening methods under virus-free conditions are still urgent needs for effective RAB inhibitors discovery.
文献关键词：
中图分类号：
作者姓名：
Lu Miao;Wei Zhou;Chunyu Yan;Yuebin Zhang;Qinglong Qiao;Xuelian Zhou;Yingzhu Chen;Guangying Wang;Zhen-dong Guo;Jun Liu;Hailong Piao;Xia Pan;Mengxue Yan;Weijie Zhao;Guohui Li;Yueqing Li;Zhaochao Xu
作者机构：
CAS Key Laboratory of Separation Science for Analytical Chemistry,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China;State Key Laboratory of Molecular Reaction Dynamics,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China;Institute of Military Veterinary Science,The Academy of Military Medical Science of PLA,Changchun 130000,China;State Key Laboratory of Fine Chemicals,Department of Pharmaceutical Sciences,School of Chemical Engineering,Dalian University of Technology,Dalian 116024,China
文献出处：
引用格式：
[1]Lu Miao;Wei Zhou;Chunyu Yan;Yuebin Zhang;Qinglong Qiao;Xuelian Zhou;Yingzhu Chen;Guangying Wang;Zhen-dong Guo;Jun Liu;Hailong Piao;Xia Pan;Mengxue Yan;Weijie Zhao;Guohui Li;Yueqing Li;Zhaochao Xu-.Rapid screening of SARS-CoV-2 inhibitors via ratiometric fluorescence of RBD—ACE2 complexes in living cells by competitive binding)[J].药学学报（英文版）,2022(09):3739-3742
A类：
tion1,inhibitors2,proced
B类：
Rapid,screening,SARS,CoV,via,ratiometric,fluorescence,RBD,ACE2,complexes,living,cells,by,competitive,binding,To,Editor,
The,current,coronavirus,disease,pandemic,spurs,development,antiviral,drugs,number,patients,infections,continues,rise,globally,context,widespread,vaccination,Targeting,interaction,between,receptor,domain,spike,protein,host,promising,therapeutic,strategy,effectively,entry,because,strong,first,Screening,live,represented,pseudo,assays,have,been,approved,significantly,improve,effectiveness,obtained,However,they,are,greatly,limited,biosafety,level,facilities,multistep,experimental,ures,Therefore,rapid,methods,under,free,conditions,still,urgent,needs,RAB,discovery
AB值：
0.618057
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