典型文献
Danshensu alleviates pseudo-typed SARS-CoV-2 induced mouse acute lung inflammation
文献摘要：
Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)can induce acute inflammatory response like acute lung inflammation(ALI)or acute respiratory distress syndrome,leading to severe progression and mortality.Therapeutics for treatment of SARS-CoV-2-triggered respiratory inflammation are urgent to be discovered.Our previous study shows that Salvianolic acid C potently inhibits SARS-CoV-2 infection.In this study,we investigated the antiviral effects of a Salvia miltiorrhiza compound,Danshensu,in vitro and in vivo,including the mechanism of S protein-mediated virus attachment and entry into target cells.In authentic and pseudo-typed virus assays in vitro,Danshensu displayed a potent antiviral activity against SARS-CoV-2 with EC50 of 0.97 μM,and potently inhibited the entry of SARS-CoV-2 S protein-pseudo-typed virus(SARS-CoV-2 S)into ACE2-overexpressed HEK-293T cells(IC50=0.31 μM)and Vero-E6 cell(IC50=4.97 μM).Mice received SARS-CoV-2 S via trachea to induce ALI,while the VSV-G treated mice served as controls.The mice were administered Danshensu(25,50,100 mg/kg,i.v.,once)or Danshensu(25,50,100mg-kg-1·d-1,oral administration,for 7 days)before SARS-CoV-2 S infection.We showed that SARS-CoV-2 S infection induced severe inflammatory cell infiltration,severely damaged lung tissue structure,highly expressed levels of inflammatory cytokines,and activated TLR4 and hyperphosphorylation of the NF-κB p65;the high expression of angiotensinogen(AGT)and low expression of ACE2 at the mRNA level in the lung tissue were also observed.Both oral and intravenous pretreatment with Danshensu dose-dependently alleviated the pathological alterations in mice infected with SARS-CoV-2 S.This study not only establishes a mouse model of pseudo-typed SARS-CoV-2(SARS-CoV-2 S)induced ALI,but also demonstrates that Danshensu is a potential treatment for COVID-19 patients to inhibit the lung inflammatory response.
文献关键词：
中图分类号：
作者姓名：
Wei Wang;Sha-sha Li;Xin-feng Xu;Chan Yang;Xiao-ge Niu;Shu-xian Yin;Xiao-yan Pan;Wei Xu;Guo-dong Hu;Chan Wang;Shu-wen Liu
作者机构：
Guangdong Provincial Key Laboratory of New Drug Screening,School of Pharmaceutical Sciences,Southern Medical University,Guangzhou 510515,China;Department of Respiratory and Critical Care Medicine,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China;State Key Laboratory of Virology,Wuhan Institute of Virology,Center for Biosafety Mega-Science,Chinese Academy of Sciences,Wuhan 430071,China;State Key Laboratory of Organ Failure Research,Guangdong Provincial Institute of Nephrology,Southern Medical University,Guangzhou 510515,China
文献出处：
引用格式：
[1]Wei Wang;Sha-sha Li;Xin-feng Xu;Chan Yang;Xiao-ge Niu;Shu-xian Yin;Xiao-yan Pan;Wei Xu;Guo-dong Hu;Chan Wang;Shu-wen Liu-.Danshensu alleviates pseudo-typed SARS-CoV-2 induced mouse acute lung inflammation)[J].中国药理学报（英文版）,2022(04):771-780
A类：
Danshensu
B类：
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AB值：
0.502596
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