Objective:Plant hormones act as chemical messengers in the regulation of plant development and meta-bolism.The production of ginsenosides in Panax hybrid is promoted by auxins that are transported and accumulated by PIN-FORMED(PIN)and PIN-LIKES(PILS)auxin transporters.However,genome-wide studies of PIN/PILS of ginseng are still scarce.In current study,identification and transcriptional profiling of PIN/PILS gene families,as well as their potential relationship with ginsenoside biosynthesis in Panax ginseng were investigated.Methods:PIN/PILS genes in P.ginseng was identified via in silico genome-wide analysis,followed by phy-logenetic relationships,gene structure,and protein profiles investigation.Moreover,previously reported RNA-sequence data from various tissues and roots after infection were utilized for PIN/PILS genes expres-sion pattern analysis.The Pearson's correlation analysis of specific PIN/PILS genes expression level and main ginsenoside contents were taken to reveal the potential relationship between auxin transports and ginsenoside biosynthesis in P.ginseng.Results:A genome-wide search of P.ginseng genome for homologous auxin transporter genes identified a total of 17 PIN and 11 PILS genes.Sequence alignment,putative motif organization,and sub-cellular local-ization indicated redundant and complementary biological functions of these PIN/PILS genes.Most PIN/PILS genes were differentially expressed in a tissue-specific manner,and showed significant correlations with ginsenoside content correspondingly.Eight auxin transporter genes,including both PIN and PILS subfamily members,were positively correlated with ginsenoside content(cor＞0.60;P-value＜0.05).The expression levels of eleven auxin transporter genes were increased dramatically in the early stage(0-0.5 DPI)after Cylindrocarpon destructans infection,accompanied with various overall expression pat-terns,implying the dynamic auxin transport in response to biotic stress.Conclusion:Based on the results,we speculate that the accumulation or depletion in temporal or spatial manner of auxin by PIN/PILS transporters involved in the regulation of HMGR activity and subsequent ginsenoside biosynthesis.

Shuiming Xiao;Yang Chu;Yanjun Chen;Qinghe Zhao;Baosheng Liao;Jingjing Zhang;Yuan Gao;Jiang Xu;Shilin Chen

Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;College of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430065,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193,China

中草药（英文版）

[1]Shuiming Xiao;Yang Chu;Yanjun Chen;Qinghe Zhao;Baosheng Liao;Jingjing Zhang;Yuan Gao;Jiang Xu;Shilin Chen-.Genome-wide identification and transcriptional profiling analysis of PIN/PILS auxin transporter gene families in Panax ginseng)[J].中草药（英文版）,2022(01):48-57

PILS,auxins,FORMED,LIKES,Cylindrocarpon,destructans

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