典型文献
Integrative SMRT sequencing and ginsenoside profiling analysis provide insights into the biosynthesis of ginsenoside in Panax quinquefolium
文献摘要：
Panax quinquefolium is one of the most common medicinal plants worldwide.Ginsenosides are the major pharmaceut-ical components in P.quinquefolium.The biosynthesis of ginsenosides in different tissues of P.quinquefolium remained largely un-known.In the current study,an integrative method of transcriptome and metabolome analysis was used to elucidate the ginsenosides biosynthesis pathways in different tissues of P.quinquefolium.Herein,22 ginsenosides in roots,leaves,and flower buds showed un-even distribution patterns.A comprehensive P.quinquefolium transcriptome was generated through single molecular real-time(SMRT)and second-generation sequencing(NGS)technologies,which revealed the ginsenoside pathway genes and UDP-glycosyltransferases(UGT)family genes explicitly expressed in roots,leaves,and flower buds.The weighted gene co-expression network analysis(WGCNA)of ginsenoside biosynthesis genes,UGT genes and ginsenoside contents indicated that three UGT genes were positively correlated to pseudoginsenoside F11,notoginsenoside R1,notoginsenoside R2 and pseudoginsenoside RT5.These results provide in-sights into ginsenoside biosynthesis in different tissues of P.quinquefolium.
文献关键词：
中图分类号：
作者姓名：
DI Peng;YAN Yan;WANG Ping;YAN Min;WANG Ying-Ping;HUANG Lu-Qi
作者机构：
State Key Laboratory Breeding Base of Dao-Di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Center for Post-doctoral Research,China Academy of Chinese Medical Sciences,Beijing 100700,China;State Local Joint Engineering Research Center of Ginseng Breeding and Application,Jilin Agricultural University,Changchun 130118,China
文献出处：
引用格式：
[1]DI Peng;YAN Yan;WANG Ping;YAN Min;WANG Ying-Ping;HUANG Lu-Qi-.Integrative SMRT sequencing and ginsenoside profiling analysis provide insights into the biosynthesis of ginsenoside in Panax quinquefolium)[J].中国天然药物,2022(08):614-626
A类：
Ginsenosides,pharmaceut,pseudoginsenoside,RT5
B类：
Integrative,SMRT,sequencing,profiling,analysis,provide,insights,into,biosynthesis,Panax,quinquefolium,most,common,medicinal,plants,worldwide,are,major,ical,components,ginsenosides,different,tissues,remained,largely,un,known,current,study,integrative,method,transcriptome,metabolome,was,used,elucidate,pathways,Herein,roots,leaves,flower,buds,showed,even,distribution,patterns,comprehensive,generated,through,single,molecular,real,second,generation,NGS,technologies,which,revealed,genes,UDP,glycosyltransferases,UGT,family,explicitly,expressed,weighted,expression,network,WGCNA,contents,indicated,that,three,were,positively,correlated,F11,notoginsenoside,R1,These,results
AB值：
0.484876
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