典型文献
The mechanism by which hyperbaric oxygen treatment alleviates spinal cord injury:genome-wide transcriptome analysis
文献摘要：
Accumulating studies have demonstrated that hyperbaric oxygen(HBO)treatment alleviates spinal cord injury(SCI).However,the underlying mechanism by which HBO alleviates SCI remains to be elucidated.In this study,we performed genome-wide transcriptional profiling of the spinal cord between SCI mice and mice that received HBO treatment by high-throughput RNA sequencing at 1 week after SCI.We also compared genome-wide transcriptional profiles from SCI mice and sham-operated mice.We found 76 differentially co-expressed genes in sham-operated mice,SCI mice,and HBO-treated SCI mice.Using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis,we identified the biological characteristics of these differentially expressed genes from the perspectives of cell component,biological process,and molecular function.We also found enriched functional pathways including ferroptosis,calcium signaling pathway,serotonergic synapse,hypoxia-inducible factor-1 signaling pathway,cholinergic synapse,and neuroactive ligand-receptor interaction.We performed quantitative reverse transcription-polymerase chain reaction and validated that HBO treatment decreased the expression of Hspb1(heat shock protein beta 1),Hmox1(heme oxygenase 1),Ftl1(ferritin light polypeptide 1),Tnc(tenascin C)and Igfbp3(insulin-like growth factor binding protein 3)and increased the expression of Slc5o7(solute carrier family 5 choline transporter member 7)after SCI.These results revealed the genome-wide transcriptional profile of the injured spinal cord after HBO treatment.Our findings contribute to a better understanding of the mechanism by which HBO treats SCI and may provide new targets for SCI intervention.
文献关键词：
中图分类号：
作者姓名：
Zhen-Cheng Sun;Fang Liang;Jing Yang;Yong Hai;Qing-Jun Su;Xue-Hua Liu
作者机构：
Department of Orthopedic Surgery,Beijing Chaoyang Hospital,Capital Medical University,Beijing,China;Department of Hyperbaric Oxygen,Beijing Chaoyang Hospital,Capital Medical University,Beijing,China
文献出处：
引用格式：
[1]Zhen-Cheng Sun;Fang Liang;Jing Yang;Yong Hai;Qing-Jun Su;Xue-Hua Liu-.The mechanism by which hyperbaric oxygen treatment alleviates spinal cord injury:genome-wide transcriptome analysis)[J].中国神经再生研究（英文版）,2022(12):2737-2742
A类：
Hspb1,Hmox1,Ftl1,Igfbp3,Slc5o7
B类：
mechanism,by,which,hyperbaric,treatment,alleviates,spinal,cord,injury,genome,wide,transcriptome,analysis,Accumulating,studies,have,demonstrated,that,HBO,SCI,However,underlying,remains,elucidated,In,this,study,performed,transcriptional,profiling,between,mice,received,high,throughput,sequencing,week,after,We,also,compared,profiles,from,sham,operated,found,differentially,expressed,genes,treated,Using,Ontology,Kyoto,Encyclopedia,Genes,Genomes,enrichment,identified,biological,characteristics,these,perspectives,cell,component,process,molecular,enriched,functional,pathways,including,ferroptosis,calcium,signaling,serotonergic,synapse,hypoxia,inducible,cholinergic,neuroactive,ligand,receptor,interaction,quantitative,reverse,polymerase,chain,reaction,validated,decreased,expression,heat,shock,protein,beta,heme,oxygenase,ferritin,light,polypeptide,Tnc,tenascin,insulin,like,growth,binding,increased,solute,carrier,family,transporter,member,These,results,revealed,injured,Our,findings,contribute,better,understanding,treats,may,provide,new,targets,intervention
AB值：
0.53544
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
