典型文献
EHMT2 (G9a) activation in mantle cell lymphoma and its associated DNA methylation and gene expression
文献摘要：
Objective: The function of euchromatic histone-lysine N-methyltransferase 2 (EHMT2) has been studied in several cancers; however, little is known about its role in mantle cell lymphoma (MCL). Thus, this study aimed to characterize the significance and function of EHMT2 in MCL.Methods: EHMT2 expression in MCL and reactive hyperplasia (RH) were investigated by immunohistochemistry. Genome-wide analysis of DNA methylation was performed on EHMT2 + MCL samples. The function of EHMT2 was determined by CCK8, flow cytometry, and western blot assays. Gene expression profile analysis was performed before and after EHMT2 knockdown to search for EHMT2-regulated genes. Co-immunoprecipitation (Co-IP) experiments were conducted to identify the proteins interacting with EHMT2. Results: EHMT2 was expressed in 68.57％ (24/35) of MCLs but not in any RHs. Genome-wide analysis of DNA methylation on EHMT2 + MCLs revealed that multiple members of the HOX, FOX, PAX, SOX, and CDX families were hypermethylated or hypomethylated in EHMT2 + MCLs. BIX01294, a EHMT2 inhibitor, inhibited MCL cell growth and stalled cells in the G1 phase. Additionally, BIX01294 downregulated the expressions of cell cycle proteins, cyclin D1, CDK4, and P21, but upregulated the expressions of apoptosis-related proteins, Bax and caspase-3. Co-IP experiments revealed that EHMT2 interacted with UHRF1, HDAC1, and HDAC2 but not with HDCA3. After EHMT2 knockdown, multiple genes were regulated, including CD5 and CCND1, mostly enriched in the Tec kinase signaling pathway. In addition, several genes (e.g., MARCH1, CCDC50, HIP1, and WNT3) were aberrantly methylated in EHMT2 + MCLs. Conclusions: For the first time, we determined the significance of EHMT2 in MCL and identified potential EHMT2-regulated genes.
文献关键词：
中图分类号：
作者姓名：
Jun Wang;Hui Xu;Shuang Ge;Chaoshuai Xue;Hailing Li;Xiaotong Jing;Ke Liang;Xiaoying Zhang;Cuijuan Zhang
作者机构：
Institute of Pathology and Pathophysiology,Shandong University School of Medicine,Jinan 250012,China;Department of Pathology,Qianfoshan Hospital,Jinan 250300,China;Department of Pathology,Qilu Hospital of Shandong University,Jinan 250012,China
文献出处：
引用格式：
[1]Jun Wang;Hui Xu;Shuang Ge;Chaoshuai Xue;Hailing Li;Xiaotong Jing;Ke Liang;Xiaoying Zhang;Cuijuan Zhang-.EHMT2 (G9a) activation in mantle cell lymphoma and its associated DNA methylation and gene expression)[J].癌症生物学与医学（英文版）,2022(06):836-849
A类：
euchromatic,MCLs,RHs,hypomethylated,BIX01294,HDCA3,MARCH1,CCDC50,HIP1
B类：
EHMT2,G9a,activation,mantle,lymphoma,its,associated,methylation,Objective,function,histone,lysine,methyltransferase,been,studied,several,cancers,however,little,known,about,role,Thus,this,study,aimed,characterize,significance,Methods,reactive,hyperplasia,were,investigated,by,immunohistochemistry,Genome,wide,analysis,was,performed,samples,determined,CCK8,flow,cytometry,western,blot,assays,Gene,profile,before,after,knockdown,search,genes,immunoprecipitation,experiments,conducted,identify,proteins,interacting,Results,expressed,but,not,any,revealed,that,multiple,members,HOX,FOX,PAX,SOX,CDX,families,hypermethylated,inhibitor,inhibited,growth,stalled,cells,G1,phase,Additionally,downregulated,expressions,cycle,cyclin,CDK4,P21,upregulated,apoptosis,related,Bax,caspase,interacted,UHRF1,HDAC1,HDAC2,After,including,CD5,CCND1,mostly,enriched,Tec,kinase,signaling,pathway,In,addition,WNT3,aberrantly,Conclusions,For,first,identified,potential
AB值：
0.459105
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