Objective:We aimed to establish a novel strategy for identifying key genes and active anti-inflammatory ingredients in Panax medicinal plants.Methods:First,fresh roots of 2-year-old Panax plants,including P ginseng C.A.Mey.,P quinquefolium L.,P notoginseng(Burk.)F H.Chen,P japonicus C.A.Mey.,P japonicus Mey.var.major(Burk.)C.Y.Wu et K.M.Feng,were selected as explants,and callus formation was induced under three experimental temperatures(17,24,and 30℃).Second,high-performance liquid chromatography-mass spectrometry was used to analyze the saponin content of the callus.Nitric oxide reduction efficacy was used for"component-efficacy"gray correlation analysis to find the active anti-inflammatory ingredients.Quantitative reverse-transcription polymerase chain reaction(qRT-PCR)was used to determine the inflammatory factors and verify the active ingredients'anti-inflammatory effects.Finally,qRT-PCR was used to detect the expression of key genes in the callus,and"gene-component"gray correlation analysis was used to examine the relationships between the regulatory pathway of the genes and the components.Results:Among the three experimental temperatures(17,24,and 30℃),the lowest temperature(17℃)is the most suitable for generating Panax callus.Lower-latitude native Panax notoginseng is more adaptable under high culture temperatures(24℃and 30℃)than other Panax plants.The ginsenoside contents of the callus of P notoginseng and P japonicus were the highest under similar climate conditions(17℃).Major anti-inflammatory components were G-Rh1,G-Rb1,G-Rg3,and G-Rh6/Floral-GKa.CYP76A47 contributed to the accumulation of anti-inflammatory components.Conclusions:This study provides a strategy for the gene-component-efficacy correlational study of multi-component,multi-functional,and multi-purpose plants of the same genus.

Jiao Ai;Yongshen Ren;Long Cheng;Xinqiao Liu;Yanqiu Li;Majing Jiao;Yingzhou Wang;Xin Deng;Linlin Chen

School of Pharmaceutical Science,South-Central University for Nationalities,Wuhan,Hubei,China;School of Pharmacy,Hubei University of Chinese Medicine,Wuhan,Hubei,China

针灸和草药（英文）

[1]Jiao Ai;Yongshen Ren;Long Cheng;Xinqiao Liu;Yanqiu Li;Majing Jiao;Yingzhou Wang;Xin Deng;Linlin Chen-.Identification of key genes and active anti-inflammatory ingredients in Panax medicinal plants by climate-regulated callus culture combined with gene-component-efficacy gray correlation analysis)[J].针灸和草药（英文）,2022(04):261-273

Rh6,GKa,CYP76A47

Identification,key,genes,active,inflammatory,ingredients,Panax,medicinal,by,climate,regulated,callus,culture,combined,efficacy,gray,analysis,Objective,We,aimed,establish,novel,strategy,identifying,Methods,First,fresh,roots,year,old,including,Mey,quinquefolium,notoginseng,Burk,Chen,japonicus,var,major,Wu,Feng,were,selected,explants,formation,was,induced,under,three,experimental,temperatures,Second,performance,liquid,chromatography,mass,spectrometry,used,analyze,saponin,Nitric,oxide,reduction,find,Quantitative,reverse,transcription,polymerase,chain,reaction,qRT,determine,factors,verify,effects,Finally,detect,expression,examine,relationships,between,regulatory,pathway,components,Results,Among,lowest,most,suitable,generating,Lower,latitude,native,more,adaptable,than,other,ginsenoside,contents,highest,similar,conditions,Major,Rh1,Rb1,Rg3,Floral,contributed,accumulation,Conclusions,This,study,provides,correlational,multi,functional,purpose,same,genus

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