OBJECTIVE:Yang-deficiency constitution(YADC)is a common unbalanced constitution that predisposes individuals to certain diseases.However,not all people with YADC manifest develop diseases.This calls for delineation of the underlying molecular mechanisms.Previous studies suggested that the gut microbiota and gene differential expression should be considered.METHODS:In the present study,we compared profiles of gut microbiota between four healthy YADC individuals and those of five healthy balanced constitution(BC)counterparts,based on 16S rRNA gene sequence analysis.Furthermore,YADC relevant genes identified by comparing 62 healthy YADC and 58 healthy BC individuals in total to perform intersection analysis,functional clustering and pathway enrichment analyses.RESULTS:The levels of harmful gut microbiota(Prevotellaceae,LDA score＞4.0,P=0.0141)and beneficial gut microbiota(Ruminococcaceae,LDA score＞4.0,P=0.0025,Faecalibacterium,LDA score＞4.0,P=0.0484)were both elevated in healthy YADC individuals.Also,we found that the specific metabolic pathway with 2,6-Dichloro-p-hydroquinone 1,2-Dioxygenase(PcpA)as the core in gut microbiota and the glutathione transferase activity has been enriched by YADC relevant genes in healthy YADC individuals were both responsible for the detoxification of halogenated aromatic hydrocarbon substances.CONCLUSIONS:Both beneficial and harmful factors had been detected in healthy YADC individuals,functionally,they may have triggered homeostasis to maintain the health of individuals with YADC.The homeostasis may be maintained by beneficial and harmful factors from gut flora and genes.Future studies are expected to focus on halogenated aromatic hydrocarbons and their detoxification processes.

ZHANG Huixiang;WANG Limei;GUO Jipeng;WANG Jiai;ZHANG Qianqian;WANG Yutao;LIU Xun;ZHANG Lihuan;SHI Lanlan;WU Hongxiang;CAO Xue

Institute of Neuroscience,Basic Medical College,Kunming Medical University,Kunming 650500,China;Department of Laboratory Animal Science,Kunming Medical University,Kunming 650500,China;School of Electrical and Information Technology,Yunnan Minzu University,Kunming 650500,China;School of Medicine,Kunming University,Kunming 650214,China;Basic Medical College,Kunming Medical University,Kunming 650500,China;Department of Anatomy,Histology and Embryology,Basic Medical College,Kunming Medical University,Kunming 650500,China;Faculty of Rehabilitation Medicine,Kunming Medical University,Kunming 650500,China;Department of Laboratory Animal Science,Kunming Medical University,State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Yunnan University,Kunming 650500,China

中医杂志（英文版）

[1]ZHANG Huixiang;WANG Limei;GUO Jipeng;WANG Jiai;ZHANG Qianqian;WANG Yutao;LIU Xun;ZHANG Lihuan;SHI Lanlan;WU Hongxiang;CAO Xue-.Gut microbiota and differential genes-maintained homeostasis is key to maintaining health of individuals with Yang-deficiency constitution)[J].中医杂志（英文版）,2022(01):96-101

YADC,predisposes,Dioxygenase,PcpA

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