Background::Food-borne parasitic diseases decrease food safety and threaten public health. The snail species is an intermediate host for numerous human parasitic trematodes. Orientogalba ollula has been reported as intermediate hosts of many zoonotic trematodes. Here, we investigated the prevalence of zoonotic trematodes within O. ollula in Guangxi, China, and assessed their zoonotic potential. Methods::Snails were collected from 54 sites in 9 cities throughout Guangxi. The snail and trematode larvae species were determined by combining morphological characteristics and molecular markers. The trematodes prevalence and constituent ratio were calculated and compared among different habitat environments. Phylogenetic trees of the trematode species were constructed using the neighbor-joining method with nuclear internal transcribed spacer 2 (ITS2) sequences. The developmental cycles of the isolated trematodes were examined by experimental infection in ducks. The developmental characteristics of Echinostoma revolutum was recorded by dissecting infected ducklings from 1-day post infection (dpi) to 10 dpi. Results::The overall prevalence of trematode larvae was 22.1% (1818/8238) in O. ollula from 11 sample sites. Morphological together with molecular identification, showed that E. revolutum, Australapatemon sp., Hypoderaeum conoideum, Pharyngostomum cordatum, and Echinostoma sp. parasitized O. ollula, with the highest infection rate of E. revolutum (13.0%). However, no Fasciola larvae were detected. The trematodes prevalence and constituent ratio varied in two sub-biotypes ( P < 0.01). A neighbor-joining tree analysis of ITS2 sequences resulted in distinct monophyletic clades supported by sequences from isolated larvae with high bootstrap values. Ducklings exposed to O. ollula infected with Echinostoma sp., E. revolutum, and H. conoideum larvae were successfully infected. The animal model for Echinostoma revolutum was successfully established. E. revolutum matured from larvae to adult at 10 dpi in the intestine of the duck, and the developmental characteristics of E. revolutum were characterized by the maturation of the reproductive and digestive organs at 6-8 dpi. Conclusions::This study revealed a high prevalence of zoonotic trematodes in O. ollula from Guangxi, China. Existing trematodes infection in animals and human clinical cases, coupled with the wide geographical distribution of O. ollula, necessitate further evaluations of the potential risk of spillover of zoonotic infection from animal to human and vice versa.

Orientogalba ollula;Zoonotic trematode;Intermediate host;Prevalence;ITS2

Li Jian;Ren Yijing;Yang Lei;Guo Jiani;Chen Haiying;Liu Jiani;Tian Haoqiang;Zhou Qingan;Huang Weiyi;Hu Wei;Feng Xinyu

Basic Medical College, Guangxi Traditional Chinese Medical University, Nanning 530005, Guangxi, China;College of Life Sciences, Inner Mongolia University, Hohhot 010070, China;College of Animal Science and Technology, Guangxi University, Nanning 530005, Guangxi, China;National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory of Parasite and Vector Biology of China Ministry of Health, WHO Collaborating Centre for Tropical Diseases, Joint Research Laboratory of Genetics and Ecology On Parasite-Host Interaction, Chinese Center for Disease Control and Prevention, Fudan University, 200025 Shanghai, China;Department of Infectious Diseases, Huashan Hospital, State Key Laboratory of Genetic Engineering, Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai 200438, China;School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai 20025, China;One Health Center, Shanghai Jiao Tong University-The University of Edinburgh, Shanghai 20025, China

贫困所致传染病（英文）

[1]Li Jian;Ren Yijing;Yang Lei;Guo Jiani;Chen Haiying;Liu Jiani;Tian Haoqiang;Zhou Qingan;Huang Weiyi;Hu Wei;Feng Xinyu-.A relatively high zoonotic trematode prevalence in Orientogalba ollula and the developmental characteristics of isolated trematodes by experimental infection in the animal model )[J].贫困所致传染病（英文）,2022(04):72-80

trematode,Orientogalba,ollula,trematodes,model ,Snails,Echinostoma,revolutum,ducklings,Australapatemon,Hypoderaeum,conoideum,Pharyngostomum,cordatum,Fasciola,Ducklings

relatively,zoonotic,prevalence,developmental,characteristics,isolated,by,experimental,infection,Background,Food,borne,parasitic,diseases,decrease,food,safety,threaten,public,health,snail,species,intermediate,numerous,human,has,been,reported,hosts,many,Here,investigated,within,Guangxi,China,assessed,their,potential,Methods,were,collected,from,sites,cities,throughout,larvae,determined,combining,morphological,molecular,markers,constituent,calculated,compared,among,different,habitat,environments,Phylogenetic,trees,constructed,using,neighbor,joining,method,nuclear,internal,transcribed,spacer,ITS2,sequences,cycles,examined,ducks,was,recorded,dissecting,infected,day,post,dpi,Results,overall,sample,Morphological,together,identification,showed,that,parasitized,highest,rate,However,detected,varied,two,sub,biotypes,analysis,resulted,distinct,monophyletic,clades,supported,bootstrap,values,exposed,successfully,established,matured,adult,intestine,characterized,maturation,reproductive,digestive,organs,Conclusions,This,study,revealed,Existing,animals,clinical,cases,coupled,wide,geographical,distribution,necessitate,further,evaluations,risk,spillover,vice,versa,Zoonotic,Intermediate,Prevalence

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