Background::Head and neck cancers (HNCs) are a heterogeneous group of tumors that progress owing to varied enviromental and genetic risk factors. Viral infections are threatening and adept at altering the expression of cellular transcription factors such as nuclear factor kappa B (NF-κB) and deregulation of other cellular proteins like NF kappa B inhibitor alpha (IκBα). The present study was conducted to detect high-risk genotypes of human papillomavirus (HPV) and protein expression of NF-κB signaling pathway in HNC patients with HPV infection.Methods::For HPV detection, genomic DNA from 152 HNC tumors was extracted formalin-fixed paraffin-embedded tissue DNA kit. For genotyping, polymerase chain reaction (PCR) using a general primer, HPV type-specific primers and agarose gel electrophoresis were performed. Immunohistochemistry (IHC) was also performed on 4-μm thick tissue sections using HPV E6 monoclonal antibody. Protein expression analysis of NF-κB signaling pathway including p50, p65, and IκBα was performed using IHC.Results::PCR analysis showed that 24.3% (37/152) of HNC cases were HPV positive. Among HPV positive, 86.5% (32/37) were tobacco users, while among HPV negative, 66.9% (77/115) were tobacco users. A significant association of HPV positivity and tobacco user was observed by univariate analysis [ P < 0.01; odds ratio (OR): 0.310, 95% confidence interval (CI): 0.110 to 0.870]. More HPV positive patients were with poor oral hygiene (78.3%) when compared with patients with good oral hygiene (21.6%) [ P < 0.03, OR: 2.440, 95% CI: 1.650 to 3.600]. The results of the logistic regression analysis showed that age, tobacco use and oral hygiene are significant predictors ( P < 0.02). PCR and IHC staining results confirmed that HPV16 was predominant among HNC cases (64.8%) when compared with HPV18 (35.2%). Expression of NF-κB proteins (p50, p65, and IκBα inhibitor) were also observed in HPV and non-HPV infected HNC tissues. IHC expression of p50, and p65 showed nuclear staining, while IκBα inhibitor showed cytoplasmic staining. Protein expression in HPV cases was higher as compared to HPV naive cases ( P < 0.05). Conclusions::From the study, it can be established that the use of tobacco, oral hygiene, and HPV infection may be synergistically involved in modulating the expression of NF-κB signaling pathway for the development and progression of HNC in the Pakistani population.

Human papillomavirus;NF-kappa B;p50;p65;IκBα inhibitor;Polymerase chain reaction;Immunohistochemistry;Head and neck cancer;Pakistani population

Sarwar Sumaira;Mulla Mushir;Mulla Munaz;Tanveer Rida;Sabir Maimoona;Sultan Aneesa;Malik Salman A.

Department of Biochemistry, Qauid-i-Azam University, Islamabad 45320, Pakistan;Department of Oral and Dental health, College of Applied Health Sciences in Arrass, Qassim University, Buraidah, Al Qasim 51921, Saudi Arabia;Department of Periodontology and Oral medicine, College of Dentistry, Qassim University, Buraidah, Al Qasim 51921, Saudi Arabia;Department of Microbiology, The University of Haripur, Haripur, KP 22620, Pakistan

中华医学杂志（英文版）

[1]Sarwar Sumaira;Mulla Mushir;Mulla Munaz;Tanveer Rida;Sabir Maimoona;Sultan Aneesa;Malik Salman A.-.Human papillomavirus, tobacco, and poor oral hygiene can act synergetically, modulate the expression of the nuclear factor kappa B signaling pathway for the development and progression of head and neck cancer in the Pakistani population)[J].中华医学杂志（英文版）,2022(15):1829-1836

synergetically,HNCs,enviromental,adept

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