典型文献
Mosquito larva distribution and natural Wolbachia infection in campus areas of Nakhon Ratchasima, Thailand
文献摘要：
Objective: To determine the prevalence of mosquito larvae in campus areas and the infection rate of endosymbiotic bacteria, Wolbachia in mosquito larvae.Method: The mosquito larvae samples were collected in residential areas and academic buildings of Suranaree University of Technology located in Northeastern Thailand during 2017-2018. Mosquito species identification was performed using GLOBE mosquito protocols and Rattanarithikul & Panthusiri's keys. The gene encoding for the surface protein of Wolbachia was amplified by PCR and confirmed by DNA sequencing. Results: Armigeres sp. is the highest proportion of mosquito larvae followed by Culex spp., Aedes albopictus, Aedes aegypti, and Toxorynchites spp., respectively. Aedes aegypti have breeding sites mostly in the containers found indoors, whereas the main breeding sites of Aedes albopictus were found in both outdoors and indoors. The House Index and Breteau Index for Aedes spp. was more than 5％ and 20％, respectively, in both areas, indicating that these areas are dengue sensitive. The highest proportion of Wolbachia infection was found in the larvae of Culex spp. (86.21％), followed by Aedes albopictus (69.23％) and rarely detected in Aedes aegypti (9.09％). Conclusion: The present study reported the first natural infection of Wolbachia in mosquito larvae in Thailand. Our result suggested that the mosquito species containing higher proportion of Wolbachia are less likely to be vectors for dengue. Therefore, Wolbachia transfection in mosquito larvae could be applied as a biocontrol for dengue and other mosquito-borne disease prevention.
文献关键词：
中图分类号：
作者姓名：
Thunyarat Surasiang;Sirilak Chumkiew;Pongsakorn Martviset;Pathanin Chantree;Mantana Jamklang
作者机构：
Institute of Molecular Biosciences,Mahidol University,Salaya,Nakhon Pathom,Thailand;School of Biology,Institute of Science,Suranaree University of Technology,Thailand;Research Unit in Nutraceuticals and Food Safety,Thammasat University,Pathumthani,Thailand;Department of Preclinical Science,Faculty of Medicine,Thammasat University,Pathumthani,Thailand;School of Preclinical Sciences,Institute of Science,Suranaree University of Technology,Thailand
文献出处：
引用格式：
[1]Thunyarat Surasiang;Sirilak Chumkiew;Pongsakorn Martviset;Pathanin Chantree;Mantana Jamklang-.Mosquito larva distribution and natural Wolbachia infection in campus areas of Nakhon Ratchasima, Thailand)[J].亚太热带医药杂志（英文版）,2022(07):314-321
A类：
Ratchasima,endosymbiotic,Suranaree,Rattanarithikul,Panthusiri,Armigeres,Toxorynchites,Breteau
B类：
Mosquito,distribution,natural,Wolbachia,infection,campus,areas,Nakhon,Thailand,Objective,determine,prevalence,mosquito,larvae,rate,bacteria,Method,samples,were,collected,residential,academic,buildings,University,Technology,located,Northeastern,during,species,identification,was,performed,using,GLOBE,protocols,keys,gene,encoding,surface,protein,amplified,by,confirmed,sequencing,Results,highest,proportion,followed,Culex,spp,Aedes,albopictus,aegypti,respectively,have,breeding,sites,mostly,containers,found,indoors,whereas,main,both,outdoors,House,Index,more,than,indicating,that,these,dengue,sensitive,rarely,detected,Conclusion,present,study,reported,first,Our,result,suggested,containing,higher,less,likely,be,vectors,Therefore,transfection,could,applied,biocontrol,other,borne,disease,prevention
AB值：
0.436495
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