Mesenchymal stem cells(MSCs)have attracted great attention as a source of cells for regenerative medicine owing to their self-renewal ability and multiple differentiation potential.However,the serious shortage of MSCs and the difficulty of maintaining the differentiation potential during culture in vitro limit their application.Microcarrier culture technology is an effective method to realize large-scale culture of MSCs,whereas the effect of microcarriers properties on MSCs proliferation and differentiation potential maintenance should be investigated.In this study,Konjac glucomannan(KGM)microcarriers with a wide range of rigidity from 0.2 MPa to 10 MPa were prepared using water-in-oil emulsion polymerization.It was found that the microcarriers rigidity had great influence on MSCs by regulating cell spreading rate.Rapid proliferation and good differentiation potential maintenance of MSCs were achieved on the intermediate rigidity about 2.51±0.65 MPa with the faster spreading rate of cells.As a result,MSCs on 2.51±0.65 MPa KGM microcarriers proliferated about 27 times,which was 1.7 times as much as MSCs cultured on the commercial microcarriers Cytodex-1.The differentiation potential was also improved about 3.2 times compared to Cytodex-1.Therefore,it is indispensable to regulate microcarriers rigidity,especially for application of MSCs.

Xing-Ran Yan;Juan Li;Xiang-Ming Na;Tong Li;Yu-Fei Xia;Wei-Qing Zhou;Guang-Hui Ma

State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China;University of Chinese Academy of Sciences,Beijing 100049,China

高分子科学（英文版）

[1]Xing-Ran Yan;Juan Li;Xiang-Ming Na;Tong Li;Yu-Fei Xia;Wei-Qing Zhou;Guang-Hui Ma-.Mesenchymal Stem Cells Proliferation on Konjac Glucomannan Microcarriers:Effect of Rigidity)[J].高分子科学（英文版）,2022(09):1080-1089

Glucomannan,Microcarriers,Rigidity,Microcarrier,microcarriers

Mesenchymal,Stem,Cells,Proliferation,Konjac,Effect,stem,cells,MSCs,have,attracted,great,attention,source,regenerative,medicine,owing,their,self,renewal,ability,multiple,differentiation,potential,However,serious,shortage,difficulty,maintaining,during,vitro,limit,application,technology,effective,method,realize,large,scale,whereas,properties,proliferation,maintenance,should,be,investigated,In,this,study,glucomannan,KGM,wide,range,rigidity,from,were,prepared,using,water,oil,emulsion,polymerization,It,was,found,that,had,influence,by,regulating,spreading,Rapid,good,achieved,intermediate,about,faster,result,proliferated,times,which,much,cultured,commercial,Cytodex,also,improved,compared,Therefore,indispensable,regulate,especially

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