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典型文献
A simple AuNPs-based colorimetric aptasensor for chlorpyrifos detection
文献摘要:
In this work,a simple gold nanoparticles(AuNPs)based colorimetric biosensor was developed for chlor-pyrifos(Chl)detection using an aptamer as the capture probe.The Chl-aptamer with low dissociation constant(Kd)of 58.59±6.08 nmol/L was selected by ssDNA library immobilized systematic evolution of ligands by enrichment(SELEX).In the absence of Chl,the Chl-aptamer acted as the stabilizer for AuNPs in salt solution.In the presence of Chl,the highly specific Chl-aptamer bound with Chl targets immediately,thus a self-aggregation of AuNPs induced by salt was displayed.The fabricated colorimetric aptasensor exhibited an excellent sensitivity for Chl detection with the LOD as low as 14.46 nmol/L.In addition,the aptasensor was applied to test Chl in tap water,cucumber and cabbage samples,the excellent recoveries with acceptable RSD values below 5%demonstrated that the method can be considered as a promising tool for simple,rapid Chl detection.
文献关键词:
作者姓名:
Yuan Liu;Taotao Li;Gaojian Yang;Yan Deng;Xianbo Mou;Nongyue He
作者机构:
State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096,China;Hunan Provincial Key Lab of Dark Tea and Jin-Hua,School of Materials and Chemical Engineering,Hunan City University,Yiyang 413000,China;Hunan Key Laboratory of Biomedical Nanomaterials and Devices,Hunan University of Technology,Zhuzhou 412007,China;Medical School of Ningbo University,Ningbo 315211,China
引用格式:
[1]Yuan Liu;Taotao Li;Gaojian Yang;Yan Deng;Xianbo Mou;Nongyue He-.A simple AuNPs-based colorimetric aptasensor for chlorpyrifos detection)[J].中国化学快报(英文版),2022(04):1913-1916
A类:
pyrifos
B类:
simple,AuNPs,colorimetric,aptasensor,chlorpyrifos,detection,In,this,work,gold,nanoparticles,biosensor,was,developed,Chl,using,aptamer,capture,probe,dissociation,constant,Kd,nmol,selected,by,ssDNA,library,immobilized,systematic,evolution,ligands,enrichment,SELEX,absence,acted,stabilizer,salt,solution,presence,highly,specific,bound,targets,immediately,thus,self,aggregation,induced,displayed,fabricated,exhibited,excellent,sensitivity,LOD,addition,applied,test,tap,water,cucumber,cabbage,samples,recoveries,acceptable,RSD,values,below,demonstrated,that,method,can,considered,promising,tool,rapid
AB值:
0.549974
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